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Hussein, A., El-Sheikh, S., Darwish, Z., Hussein, K., Gaafar, A. (2018). EFFECT OF GENISTEIN AND OXALIPLATIN ON CANCER STEM CELLS IN ORAL SQUAMOUS CELL CARCINOMA: AN EXPERIMENTAL STUDY. Alexandria Dental Journal, 43(1), 117-123. doi: 10.21608/adjalexu.2018.57611
Ahmed M. Hussein; Sahar M. El-Sheikh; Zeinab E. Darwish; Khaled A. Hussein; Ashraf I. Gaafar. "EFFECT OF GENISTEIN AND OXALIPLATIN ON CANCER STEM CELLS IN ORAL SQUAMOUS CELL CARCINOMA: AN EXPERIMENTAL STUDY". Alexandria Dental Journal, 43, 1, 2018, 117-123. doi: 10.21608/adjalexu.2018.57611
Hussein, A., El-Sheikh, S., Darwish, Z., Hussein, K., Gaafar, A. (2018). 'EFFECT OF GENISTEIN AND OXALIPLATIN ON CANCER STEM CELLS IN ORAL SQUAMOUS CELL CARCINOMA: AN EXPERIMENTAL STUDY', Alexandria Dental Journal, 43(1), pp. 117-123. doi: 10.21608/adjalexu.2018.57611
Hussein, A., El-Sheikh, S., Darwish, Z., Hussein, K., Gaafar, A. EFFECT OF GENISTEIN AND OXALIPLATIN ON CANCER STEM CELLS IN ORAL SQUAMOUS CELL CARCINOMA: AN EXPERIMENTAL STUDY. Alexandria Dental Journal, 2018; 43(1): 117-123. doi: 10.21608/adjalexu.2018.57611

EFFECT OF GENISTEIN AND OXALIPLATIN ON CANCER STEM CELLS IN ORAL SQUAMOUS CELL CARCINOMA: AN EXPERIMENTAL STUDY

Article 21, Volume 43, Issue 1, April 2018, Page 117-123  XML PDF (816.01 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2018.57611
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Authors
Ahmed M. Hussein1; Sahar M. El-Sheikh2; Zeinab E. Darwish3; Khaled A. Hussein4; Ashraf I. Gaafar5
1Assistant Lecturer of Oral and Maxillofacial Pathology Department, Faculty of Oral and Dental Medicine, SVU, Kena, Egypt.
2Vice Dean of Student’s Affair, Professor of Oral Pathology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
3Head of Oral Pathology Department, Professor of Oral Pathology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
4Professor of Oral Pathology, Department of Oral Pathology, Faculty of Dental Medicine, Al-Azhar University, Assiut, Egypt.
5Associate Professor of Oral Pathology, Department of Oral Pathology, Faculty of Dental Medicine, Al-Azhar University, Assiut, Egypt.
Abstract
INTRODUCTION: Squamous cell carcinoma is one of the malignant diseases that affect the oral cavity worldwide. One of the theories regarding oral carcinogenesis is that tumor growth is dependent on cancer stem cells. Markers specific for these cells as CD44 have been investigated in hope of developing a deeper understanding for their role in carcinogenesis. Genistein, as chemopreventive agent, has been shown to suppress the growth of several tumors. Oxaliplatin is a chemotherapeutic compound that did show a range of antitumor activity. OBJECTIVES: This research was carried out to study the effect of genistein, oxaliplatin either alone or in combination during experimentally DMBA induced hamster buccal pouch carcinogenesis using CD44 antibody as a marker. MATERIALS AND METHODS: A total of 100 young Syrian hamsters distributed into groups as follows: 4 normal animals examined for the histology of the normal pouch mucosa and 96 animals divided into; group I, as a control group, in which pouches were painted with a heavy mineral oil only; group II were painted with DMBA mixed in a heavy mineral oil. These animals were randomly divided into 4 subgroups as following: group IIA only painted with DMBA; group IIB where genistein was orally administrated; group IIC were injected with oxaliplatin; and group IID in which both genistein and oxaliplatin were given. RESULTS: Both genistein and oxaliplatin provided a significant reduction in carcinogenesis process of DMBA induced oral squamous cell carcinoma. Moreover, they provided a significant decrease in the proliferation and activity of cancer stem cells as measured by the CD44 antibody. CONCLUSIONS: Genistein provides a chemoprevention role and the oxaliplatin produces a chemotherapeutic effect during the process of carcinogenesis. The combined action of both agents was better than the effect of each agent alone.
Keywords
genistein; Oxaliplatin; Cancer stem cells; CD44
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