Home Articles List Article Information
Alexandria Dental Journal
Journal Archive
Volume Volume 49 (2024)
Volume Volume 48 (2023)
Volume Volume 47 (2022)
Volume Volume 46 (2021)
Volume Volume 45 (2020)
Volume Volume 44 (2019)
Volume Volume 43 (2018)
Volume Volume 42 (2017)
Volume Volume 41 (2016)
Volume Volume 40 (2015)
Abd El-Wahed, A., Hassan, M., El-Hossary, W., Korraah, A. (2018). LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY). Alexandria Dental Journal, 43(3), 81-87. doi: 10.21608/adjalexu.2018.58003
Asmaa M Abd El-Wahed; Magda M Hassan; Wafaa H El-Hossary; Ahmed M Korraah. "LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY)". Alexandria Dental Journal, 43, 3, 2018, 81-87. doi: 10.21608/adjalexu.2018.58003
Abd El-Wahed, A., Hassan, M., El-Hossary, W., Korraah, A. (2018). 'LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY)', Alexandria Dental Journal, 43(3), pp. 81-87. doi: 10.21608/adjalexu.2018.58003
Abd El-Wahed, A., Hassan, M., El-Hossary, W., Korraah, A. LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY). Alexandria Dental Journal, 2018; 43(3): 81-87. doi: 10.21608/adjalexu.2018.58003

LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY)

Article 14, Volume 43, Issue 3, December 2018, Page 81-87  XML PDF (860.38 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2018.58003
View on SCiNiTO View on SCiNiTO
Authors
Asmaa M Abd El-Wahed1; Magda M Hassan2; Wafaa H El-Hossary3; Ahmed M Korraah4
1Demonstrator in Oral Pathology department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
2Acting Head of Oral Pathology Department, Professor of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
3- Lecturer of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
4Lecturer of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
Abstract
Introduction: Thymoquinone is one of the phytochemicals used as chemopreventive agents for oral cancer control. TQ loaded on nanoparticles had shown higher anti-proliferative and anti-inflammatory effects than free TQ. Natural products that can inhibit Cyclooxygenase-2 (COX-2) would be a better choice in the prevention of tumor development with fewer side effects than pharmaceutical agents. Objective: To study the expression of Cox-2 in HBP/DMBA carcinogenesis model following combined topical application of TQ loaded on gold nanoparticles on alternative days. Material and Methods: This research was carried out on archival paraffin blocks of a previous thesis. The paraffin blocks represented hamster buccal pouches (HBPs) from 85 male Syrian golden hamsters were divided into 2 groups. The control groups included: I: negative control group, scarified at day zero then after 7 and 14 weeks, and II: positive control, treated with 7, 12-Dimethylbenz [a]-anthracene (DMBA) for 7 and 14 weeks. The experimental groups included: (III, IV and V) which were treated with the chemopreventive agents for 2 weeks then combined DMBA and the chemopreventive agents on alternative days for 7 and 14 weeks. Sections of 5µm were cut and processed for H&E and IHC stains for light microscopic study. Results: Negative and self-control groups showed negative COX-2 immunoreactivity. Group II showed intense Cox-2 immunoreactivity at both 7 and 14 weeks. Groups; III and IV showed moderate and intense Cox-2 immunoreactivity at 7 and 14 weeks, respectively. Group V showed mild and moderate Cox-2 staining reaction at 7 and 14 weeks, respectively. Conclusion: Topical application of G-NPs-TQ (0.001) for 7 and 14 weeks was able to reduce Cox-2 expression and in turn retard the carcinogenesis process due to its anti-inflammatory effect.
Keywords
Thymoquinone; Gold-nanoparticles; Oral squamous cell carcinoma; Cyclooxygenase-2; and Dimethylbenz-(a) anthracene
References
  1. El-Mofty S. Early detection of oral cancer. Egypt J Oral Maxillofac Surg.2010; 1:25-31.
  2. Radio L, Luce D. A review of risk factors for oral cavity cancer: the importance of a standardized case definition. Commun Dent Oral Epidemiol.2013; 41(2):97-109.
  3. Fotedar V, Fotedar S, Seam RK, Gupta MK. Oral cancer and chemoprevention. Int J Pharm Sci Invent.2013;2(2):16-20.
  4. Attoub S, Sperandio O, Raza H, Arafat A, Al-Salam S, Al Sultan MA. Thymoquinone as an anticancer agent: Evidence from inhibition of cancer cells viability and invasion in vitro and tumor growth in vivo. Fund Clin Pharmacol.2013; 27(5):557-69.
  5. Fararh KM, Atoji Y, Shimizu Y, Shiina T, Nikami H. Mechanisms of the hypoglycaemic and immunopotentiating effects of Nigella sativa L. oil in streptozotocin-induced diabetic hamsters. Res Vet Sci.2004; 77(2):123-29.
  6. Woo CC, Kumar AP, Sethi G, Tan KH. Thymoquinone: Potential cure for inflammatory disorders and cancer. Biochem Pharmacol.2012; 83(4):443-51.
  7. Gao Wm, Xu K, Ji L, Tang B. Effect of gold nanoparticles on glutathione depletion-induced hydrogen peroxide generation and apoptosis in HL7702 cells. Toxicol Lett.2011; 205(1): 86-95.
  8. Guo Q, Yuan J, Zeng J. Biosynthesis of gold nanoparticles using a kind of flavonol: Dihydromyricetin. Colloids Surf A Physicochem Eng Asp.2014; 441:127-32.
  9. Sakoda LC, Gao YT, Chen BE, Chen J, Rosenberg PS, Rashid A, et al. Prostaglandin-endoperoxide synthase 2 (PTGS2) gene polymorphisms and risk of biliary tract cancer and gallstones: a population-based study in Shanghai, China. Carcinogenesis.2006; 7(6):1251-56.
  10. Zaoui A, Cherrah Y, Mahassini N, Alaoui K, Amarouch H, Hassar M. Acute and chronic toxicity of Nigella sativa fixed oil.Phytomed.2002;9(1): 69-74.
  11. Mantovani A, Allavena P, Sica A, Balkwill F. Cancerrelated inflammation. Nature.2008; 454(7203):436-44.
  12. Kinzler KW, Morin PJ, Vogelstein B. Chan TA. Mechanisms underlying nonsteroidal antiinflammatory drug-mediated apoptosis. Proc Natl Acad Sci USA. 1998; 95(2):681-86.
  13. Xu D, Ma Y, Zhao B, Li S, Zhang Y, Pan S, et al. Thymoquinone induces G2/M arrest, inactivates PI3K/Akt and nuclear factor-kappa B pathways in human cholangiocarcinomas both in vitro and in vivo. Oncol Rep.2014; 31(5):2063-70.
  14. Al-Wafai RJ. Nigella sativa and thymoquinone suppress cyclooxygenase-2 and oxidative stress in pancreatic tissue of streptozotocin-induced diabetic rats. Pancreas.2013; 42(5):841-49.
  15. Minter HA, Eveson JW, Huntley S, Elder DJ, Hague A. The cyclooxygenase 2-selective inhibitor NS398 inhibits proliferation of oral carcinoma cell lines by mechanisms dependent and independent of reduced prostaglandin E2 synthesis. Clin Ca Res.2003; 9(5):1885-97.
  16. Shata MS, Hassan MM, Abou El-Nour KM, El-Azab MF. Nano-chemoprevention of oral squamous carcinoma using thymoquinone loaded on gold nanoparticles. Proc. ICNA-III. Feb.22-25.2016; Hurghada. Egypt.
  17. El-Dakhakhny M, Hassan MA, Abdel-Aziz G. Effect of thymoquinone and poly-thymoquinone on chemicallyinduced oral epithelial dysplasia (experimental study), (Part I).Intern J Acad Res.2009;1(2):107-17.
  18. Banoczy J, Csiba A. Occurrence of epithelial dysplasia in oral leukoplakia. Analysis and follow-up study of 12 cases. Oral Surg Oral Med Oral Pathol.1976;42(6):766-74.
  19. Pindborg JJ, Reichart P, Smith CJ, Van der Waal I. Histological typing of cancer and precancer of the oral mucosa, 2nd ed. Springer.;pp21-26. 1997 http://www.springer.com/series/1174
  20. Mane DR, Kale AD, Belaldavar C. Validation of immunoexpression of tenascin-C in oral precancerous and cancerous tissues using ImageJ analysis with novel immunohistochemistry profiler plugin: An immunohistochemical quantitative analysis. J Oral Maxillofac Pathol. 2017; 21(2):211-7.
  21. Sawhney M, Rohatgi N, Kaur J, Shishodia S, Sethi G, Gupta SD et al. Expression of NF-κB parallels COX-2 expression in oral precancer and cancer: Association with smokeless tobacco. Int J Ca.2007; 120(12):2545-56.
  22. Hassan MMA. El-Dakhakhny MI. Effect of some Nigella sativa L. constituents on chemical carcinogenesis in hamster cheek pouch. J Egypt Soc Pharmacol& Exp Ther, 1992; 11(2): 675- 91.
  23. AL-Jawfi KAM, Hassan MMA, El-Gohary AM. Effect of Nigella sativa oil on the hamster lymphocytes secondary to DMBA-induced carcinogenesis. Suez Canal Med J.2008;11(1):75-80.
  24. Rajkamal G, Suresh K, Sugunadevi G, Vijayaanand MA, Rajalingam K. Evaluation of chemopreventive effects of thymoquinone on cell surface glycoconjugates and cytokeratin expression during DMBA induced hamster buccal pouch carcinogenesis. Biochem Mol Biol Rep.2010; 43(10):664-69.
  25. Banerjee S, Kaseb AO, Wang Z, Kong D, Mohammad M et al. Antitumor activity of gemcitabine and oxaliplatin is augmented by thymoquinone in pancreatic cancer.Ca Res.2009;69(13):5575-83.
  26. Nagini S, Letchoumy PV, Thangavelu A, Ramachandran Cr. Of humans and hamsters: a comparative evaluation of carcinogen activation, DNA damage, cell proliferation, apoptosis, invasion, and angiogenesis in oral cancer patients and hamster buccal pouch carcinomas. Oral Oncol.2009; 45(6):31-37.
  27. Anto RJ, Mukhopadhyay A, Shishodia S, Gairola CG, Aggarwal BB. Cigarette smoke condensate activates nuclear transcription factor-kappa B through phosphorylation and degradation of I kappa B (alpha): Correlation with induction of cyclooxigenase2.Carcinogenesis.2002; 23(9):1511-18.
  28. Jeng JH, Wang YJ, Chiang BL. Roles of keratinocyte inflammation in oral cancer: Regulating the prostaglandin E2, interleukin-6 and TNF-α production of oral epithelial cells by areca nut extract and arecoline.Carcinogenesis.2003;24:1301-15.
  29. Sharma C, Kaur J, Shishodia S, Aggarwal BB, Ralhan R. Curcumin down regulates smokeless tobacco-induced NF-κB activation and COX-2 expression in human oral premalignant and cancer cells.Toxicol.2006;228:1-15.
  30. Shibata M, Kodani I, Osaki M, Araki K, Adachi H, Ryoke K. Cyclo-oxygenase-1 and -2 expression in human oral mucosa, dysplasias and squamous cell carcinomas and their pathological significance. Oral Oncol.2005;41:304- 12.
  31. Sudbo J, Ristimaki A, Sondresen JE. Cyclooxygenase-2 (COX-2) expression in high-risk premalignant oral lesions. Oral Oncol.2003; 39:497-505.
  32. Renkonen J, Wolff H, Paavonen T. Expression of cyclooxygenase- 2 in human tongue carcinoma and its precursor lesions. Virchows Arch.2002; 440: 594-97
  33. Shamma A, Yamamoto H, Doki Y, Okami J, Kondo M, Fujiwara Y. Up-regulation of cyclooxygenase 2 in squamous carcinogenesis of the esophagus. Clin Ca Res.2000; 6:1229-38.
  34. Frank G, Juhn SK, Adams GL. Inhibition of head and neck tumor cell growth with arachidonic acid metabolism inhibition.Laryngoscope.1996;106(2):129-34.
  35. Prakobwong S, Khoontawad J, Yongvanit P, Pairojkul C et al. Curcumin decreases cholangio-carcinogenesis in hamsters by suppressing inflammation-mediated molecular events related to multistep carcinogenesis. Int J Ca.2011; 129(1):88-100.
  36. Maher SG, Reynolds JV. Basic concepts of inflammation and its role in carcinogenesis. Recent Results Ca Res.2011; 185:1-34.
  37. Masferrer JL, Leahy KM, Koki AT, Zweifel BS et al. Antiangiogenic and antitumor activities of cyclooxygenase-2 inhibitors.Ca Res.2000;60:1306-11.
Statistics
Article View: 201
PDF Download: 478