Aboulgheit, S., Abdelkader, S., Aboushelib, M., Mehanna, R., Omar, E., Ibrahim, Y. (2021). COLLAGEN CHITOSAN SCAFFOLDS ON INDUCED SKIN DEFECT IN A RAT MODEL (AN EXPERIMENTAL STUDY). Alexandria Dental Journal, 46(Issue 1), 136-143. doi: 10.21608/adjalexu.2021.144867
Salma A. Aboulgheit; Sally M. Abdelkader; Moustafa N. Aboushelib; Radwa A. Mehanna; Enas M. Omar; Yomna M. Ibrahim. "COLLAGEN CHITOSAN SCAFFOLDS ON INDUCED SKIN DEFECT IN A RAT MODEL (AN EXPERIMENTAL STUDY)". Alexandria Dental Journal, 46, Issue 1, 2021, 136-143. doi: 10.21608/adjalexu.2021.144867
Aboulgheit, S., Abdelkader, S., Aboushelib, M., Mehanna, R., Omar, E., Ibrahim, Y. (2021). 'COLLAGEN CHITOSAN SCAFFOLDS ON INDUCED SKIN DEFECT IN A RAT MODEL (AN EXPERIMENTAL STUDY)', Alexandria Dental Journal, 46(Issue 1), pp. 136-143. doi: 10.21608/adjalexu.2021.144867
Aboulgheit, S., Abdelkader, S., Aboushelib, M., Mehanna, R., Omar, E., Ibrahim, Y. COLLAGEN CHITOSAN SCAFFOLDS ON INDUCED SKIN DEFECT IN A RAT MODEL (AN EXPERIMENTAL STUDY). Alexandria Dental Journal, 2021; 46(Issue 1): 136-143. doi: 10.21608/adjalexu.2021.144867
COLLAGEN CHITOSAN SCAFFOLDS ON INDUCED SKIN DEFECT IN A RAT MODEL (AN EXPERIMENTAL STUDY)
1Dental Biomaterials, Faculty of Dentistry, Alexandria University, Egypt
2Professor of Dental Biomaterials, Faculty of Dentistry, Alexandria University, Egypt.
3Assistant Professor of Physiology, Faculty of Medicine, Alexandria University, Egypt.
4Lecturer of Oral Pathology Department, Faculty of Dentistry, Alexandria University, Egypt.
5Demonstrator of Dental Biomaterials, Faculty of Dentistry, Alexandria University, Egypt.
Abstract
BACKGROUND: Over the past ten years, an alternative for maxillofacial reconstruction was offered through regenerative medicine. It provided a new scope for improving the reconstruction of the oral and maxillofacial structures whether they are hard tissues which include the teeth and bone or the soft tissues like the oral mucosa, skin, nerves and blood vessels. OBJECTIVES: The aim of this study is to evaluate the effect of the collagen chitosan scaffolds on the duration and quality of skin surface healing. MATERIALS AND METHODS: An experimental in vivo comparative study was conducted, on twelve adult male Sprague-Dawley rats, where an induced skin wound was made on the backs of rats and then classified into two groups; 1-control group and 2-scaffold group. The effect of the collagen-chitosan scaffolds on the duration and quality of skin surface healing were assessed using Masson’s trichrome staining for collagen fibers and the imageJ software to calculate % area after wound healing also the number of CD 68 macrophages was counted using histological sections then the two groups were compared using student t-test (p < 0.05). RESULTS: The scaffold group showed accelerated wound healing in comparison to the control group and with a significant result. Quality of healed skin was significantly better in the scaffold treated group, which is attributed to the better collagen alignment and deposition in the stained histological sections indicated by the % area after wound healing. The number of CD 68 macrophages was higher in the scaffold group compared to the control group. CONCLUSION: The collagen chitosan scaffolds can be considered as a treatment regimen for treating skin wounds.
1. Hölzle F, Mohr C, Wolff KD. Reconstructive oral and maxillofacial surgery. Deutsches Ärzteblatt International. 2008 Nov;105(47):815. 2. Feinberg SE, Aghaloo TL, Cunningham Jr LL. Role of Tissue Engineering in Oral and Maxillofacial Reconstruction: Findings of the 2005 AAOMS Research Summit. J Oral Maxillofac Surg. 2005;63:1418- 25. 3. Wang X, Li Q, Hu X, Ma L, You C, Zheng Y, et al. Fabrication and characterization of poly(L-lactide-co-glycolide) knitted mesh-reinforced collagen-chitosan hybrid scaffolds for dermal tissue engineering. J Mech Behav Biomed Mater. 2012;8:204-15. 4. Balasubramani M, Kumar TR, Babu M. Skin substitutes: a review. Burns. 2001;27:534-44. 5. Zhao S, Li L, Wang H, Zhang Y, Cheng X, Zhou N, Rahaman MN, et al. Wound dressings composed of copper-doped borate bioactive glass microfibers stimulate angiogenesis and heal full-thickness skin defects in a rodent model. Biomaterials. 2015;53:379-91. 6. Wang X, You C, Hu X, Zheng Y, Li Q, Feng Z, Sun H, et al. The roles of knitted mesh-reinforced collagen-chitosan hybrid scaffold in the one-step repair of full-thickness skin defects in rats. Acta Biomater. 2013;9:7822-32. 7. Madhumathi K, Sudheesh Kumar PT, Abhilash S, Sreeja V, Tamura H, Manzoor K, et al. Development of novel chitin/nanosilver composite scaffolds for wound dressing applications. J Mater Sci Mater Med. 2010;21:807-13. 8. Wiegand C, Heinze T, Hipler UC. Comparative in vitro study on cytotoxicity, antimicrobial activity, and binding capacity for pathophysiological factors in chronic wounds of alginate and silver-containing alginate. Wound Repair Regen. 2009;17:511-21. 9. Burgeson RE, Nimni ME. Collagen types. Molecular structure and tissue distribution. Clin Orthop Relat Res. 1992;282:250-72. 10.Chevallay B, Herbage D. Collagen-based biomaterials as 3D scaffold for cell cultures: Applications for tissue engineering and gene therapy. Med Biol Eng Comput. 2000;38:211-8. 11.Wolf K, Alexander S, Schacht V, Coussens LM, von Andrian UH, van Rheenen J, et al. Collagen-based cell migration models in vitro and in vivo. Semin Cell Dev Biol. 2009;20:931-41. 12.Croisier F, Jérôme C. Chitosan-based biomaterials for tissue engineering. Eur Polym J. 2013;49:780-92. 13.Dhandayuthapani B, Krishnan UM, Sethuraman S. Fabrication and characterization of chitosan-gelatin blend nanofibers for skin tissue engineering. J Biomed Mater Res B Appl Biomater. 2010;94:264-72. 14.Aranaz I, Mengíbar M, Harris R, Paños I, Miralles B. Functional characterization of chitin and chitosan. Curr Chem Biol. 2009;3:203-30. 15.Yang Y, Xia T, Zhi W, Wei L, Weng J. Promotion of skin regeneration in diabetic rats by electrospun core-sheath fibers loaded with basic fibroblast growth factor. Biomaterials. 2011;32:4243-54. 16.Songjiang Z, Lixiang W. Amyloid-beta associated with chitosan nano-carrier has favorable immunogenicity and
2009;10:900-5. 17.Pacak CA, Powers JM, Cowan DB. Ultrarapid Purification of Collagen Type I for Tissue Engineering Applications. Tissue Eng Part C Methods. 2011;17:879-85. 18.de Queiroz Antonino R, Lia Fook B, de Oliveira Lima V, de Farias Rached R, Lima E, da Silva Lima R, Peniche Covas C, Lia Fook M. Preparation and characterization of chitosan obtained from shells of shrimp (Litopenaeus vannamei Boone). Marine drugs. 2017 May;15(5):141-52. 19. Han C, Zhang L, Sun J, Shi H, Zhou J, Gao C. Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering. J Zhejiang Univ Sci B. 2010;11:524-30. 20.Pereira T, Armada-da Silva PA, Amorim I, Rêma A, Caseiro AR, Gärtner A, et al. Effects of human mesenchymal stem cells isolated from Wharton's jelly of the umbilical cord and conditioned media on skeletal muscle regeneration using a myectomy model. Stem Cells Int. 2014;2014:1-16. 21.Zhao S, Li L, Wang H, Zhang Y, Cheng X, Zhou N, Rahaman MN, Liu Z, Huang W, Zhang C. Wound dressings composed of copper-doped borate bioactive glass microfibers stimulate angiogenesis and heal full-thickness skin defects in a rodent model. Biomaterials. 2015 Jun 1;53:379-91. 22.Ferreira AD, Gomes DA. Stem cell extracellular vesicles in skin repair. Bioengineering. 2019 Mar;6(1):4. 23.Chandraker A, Arscott R, Murphy G, Lian C, Bueno E, Marty F, et al. Face transplantation in a highly sensitized recipient. Mil Med. 2016;181:221-6. 24.Zahedi E, Esmaeili A, Eslahi N, Shokrgozar M, Simchi A. Fabrication and Characterization of Core-Shell Electrospun Fibrous Mats Containing Medicinal Herbs for Wound Healing and Skin Tissue Engineering. Marine drugs. 2019 Jan;17(1):27-40. 25.Blanco M, Vázquez JA, Pérez-Martín RI, G Sotelo C. Collagen Extraction Optimization from the Skin of the Small-Spotted Catshark (S. canicula) by Response Surface Methodology. Marine drugs. 2019 Jan;17(1):40. 26.Rajan N, Habermehl J, Coté MF, Doillon CJ, Mantovani D. Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications. Nat Protoc. 2006;1:2753-8. 27.Yang H, Shu Z. The extraction of collagen protein from pigskin. J Chem Pharm Res. 2014;6:683-7. 28.Pacak CA, MacKay AA, Cowan DB. An improved method for the preparation of type I collagen from skin. J Vis Exp. 2014;83:e51011. 29.Li J, Zhou C, Luo C, Qian B, Liu S, Zeng Y, et al. N-acetyl cysteine-loaded graphene oxide-collagen hybrid membrane for scarless wound healing. Theranostics. 2019;9:5839-53. 30.Mighri N, Mao J, Mighri F, Ajji A, Rouabhia M. Chitosan-coated collagen membranes promote chondrocyte adhesion, growth, and interleukin-6 secretion. Materials. 2015;8(11):7673-89. 31.Bianchera A, Salomi E, Pezzanera M, Ruwet E, Bettini R, Elviri L. Chitosan hydrogels for chondroitin sulphate controlled release: an analytical characterization. Journal of analytical methods in chemistry. 2014;2014. 32.Haifei S, Xingang W, Shoucheng W, Zhengwei M, Chuangang Y, Chunmao H. The effect of collagen-chitosan porous scaffold thickness on dermal regeneration in a one-stage grafting procedure. J Mech Behav Biomed Mater. 2014;29:114-25. 33.Romanova OA, Grigor’ev TE, Goncharov ME, Rudyak SG, Solov’yova EV, Krasheninnikov ST, Saprykin VP, Sytina EV, Chvalun SN, Pal’tsev MA, Panteleev AA. Chitosan as a modifying component of artificial scaffold for human skin tissue engineering. Bulletin of experimental biology and medicine. 2015 Aug 1;159(4):557-66. 34.Mehanna RA, Nabil I, Attia N, Bary AA, Razek KA, Ahmed TA, Elsayed F. The effect of bone marrow-derived mesenchymal stem cells and their conditioned media topically delivered in fibrin glue on chronic wound healing in rats. BioMed research international. 2015;2015.
View on SCiNiTO
Top