EFFECTIVENESS OF COMBINATION OF VITAMIN D WITH CHITOSAN HYDROXYAPATITE VERSUS CHITOSAN HYDROXYAPATITE ON BONE REGENERATION IN CRITICAL SIZE DEFECT (AN EXPERIMENTAL STUDY IN RABBITS)

Hussien, Marwa Gharib; Mohamed, Nevien; Rashad, Nagah; Metawie, Dina Mohamed Nader; El-Lakany, Sarah A

doi:10.21608/adjalexu.2024.252499.1453

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	EFFECTIVENESS OF COMBINATION OF VITAMIN D WITH CHITOSAN HYDROXYAPATITE VERSUS CHITOSAN HYDROXYAPATITE ON BONE REGENERATION IN CRITICAL SIZE DEFECT (AN EXPERIMENTAL STUDY IN RABBITS)
Articles in Press, Corrected Proof, Available Online from 02 October 2024 PDF (537.05 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2024.252499.1453
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Authors
Marwa Gharib Hussien ¹; Nevien Mohamed²; Nagah Rashad³; Dina Mohamed Nader Metawie ⁴; Sarah A El-Lakany⁵
¹Oral Surgery, Faculty of Dentistry, Alexandria University, Egypt
²Professor of Oral and Maxillofacial Surgery, Department of Oral and Maxillofacial Surgery Faculty of Dentistry, Alexandria University, Egypt
³Professor of Oral Biology, Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.
⁴Oral and Maxillofacial surgery department, Faculty of dentistry, Alexandria University, Alexandria ,Egypt.
⁵Lecturer of Industrial Pharmacy, Industrial Pharmacy Department, Faculty of Pharmacy, Alexandria University, Egypt
Abstract
Background: It is challenging for bone to heal in a critical-size lesion. Tissue engineering produces better results, but it necessitates the use of costly additives such as stem cells and bone morphogenic proteins. Vitamin D3 plays an important role in bone turnover and mineralization by means of osteoblast and osteoclast activation. Chitosan is extensively employed in bone tissue regeneration, drug delivery, wound healing and osteoconductive properpties. The use of chitosan in conjunction with other bio-active materials, such as hydroxyapatite, has demonstrated efficacy in promoting bone repair. Hydroxyapatite is a synthetic, ceramic substance that has demonstrated efficacy in the production of new bone. Injectable hydrogel has shown effectiveness in encapsulating a range of biological components, including medicines and cells for tissue engineering. Aim of the study: Evaluate and compare the amount of bone formation using chitosan hydroxyapatite composite hydrogel versus chitosan hydroxyapatite vitamin D hydrogel. Materials and Methods: 36 Male rabbits were used in the study. A critical size osseous defect was induced in the tibia of each animal. The sample was divided into 3 groups. The first group was left empty as a negative control, the second group was filled with chitosan hydroxyapatite composite hydrogel and the third group was filled with vitamin D3 chitosan hydroxyapatite hydrogel. Scarification was done after 4 and 6 weeks. Results: This study finds that vitamin D3 accelerates bone formation and promotes bone maturation. Conclusion: Applying vitamin D3 (calcitriol) locally speeded up the production of new bone as shown by histological and histomorphmetric analysis
Keywords
Chitosan; chitosan hydrogel; hydroxyapatite; Vitamin D
Main Subjects
Oral and maxillofacial surgery


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