EVALUATION OF TOPICAL OZONE THERAPY ON SOCKET HEALING IN RABBITS WITH INDUCED DIABETES MELLITUS

Document Type : Original Article

Authors

1 Demonstrator of Oral Biology, Faculty of Dentistry, Pharos University, Alexandria, Egypt

2 -Professor of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.

3 Professor of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.

Abstract

INTRODUCTION: Ozone (O3) gas is a molecule consisting of three atoms of oxygen in a dynamically unstable structure due to the presence of mesomeric states. Ozone therapy has been utilized and heavily studied for more than a century. Its effects on bone are proven and consistent. It enhance osteoblastic activity and new bone formation. Diabetes mellitus (DM) is a chronic metabolic disease that potentially affect bone metabolism, structure, and mineral density. OBJECTIVES: to evaluate histologically the possible effect of ozone therapy on bone healing after tooth extraction in rabbits with alloxan induced diabetes mellitus. MATERIALS AND METHODS Thirty adult male New Zealand rabbits weighing 1500-2500 grams (approximately six months of age) will be divided into 3 equal groups: group I;(control group), group II; (diabetic group): diabetes was induced by a single intravenous injection of alloxan in a dose of 100 mg/kg and group III:(Ozone group) rabbits was treated as in diabetic group then they was treated with ozone therapy which will be applied topically in the form of gel inside the tooth socket. Rabbits were sacrificed after six weeks. Each sample were dissected and prepared for histological evaluation. RESULTS: in diabetic group the socket showed empty central region surrounded by disorganization bony trabeculae. The bony trabeculae surrounded less vascular spaces and more fibrous tissue.in diabetic group treated with ozone therapy the socket showed complete filling of the socket with newly formed bone.The socket with newly formed bone surrounding highly vascular bone marrow. CONCLUSIONS: Ozone gel application accelerates socket healing and enhances bone formation in diabetes mellitus

Keywords

Main Subjects

References

  1. Elvis AM, Ekta JS. Ozone therapy: A clinical review. J Nat Sci Biol Med. 2011;2:66–70.
  2. Tiwari S, Avinasha A, Katiyar S, Iyer AA, Jain S. Dental applications of ozone therapy: A review of literature. Saudi J Dent Res. 2017; 8:105-11.
  3. Seidler V, Linetskiy I, Hubálková H, Stanková H, Smucler R, Mazánek J. Ozone and its usage in general medicine and dentistry: A review article. Prague Med Rep. 2008;109:5-13.
  4. Gent JF, Triche EW, Holford TR, Belanger K, Bracken MB, Beckett WS, et al. Association of low-level ozone and fine particles with respiratory symptoms in children with asthma. JAMA. 2003; 290:1859-67.
  5. Garg R, Tandon S. Ozone: A new face of dentistry. Internet J Dent Sci. 2009;2. Available at: https://www.researchgate.net/publication/215062909_Oz one_A_new_face_of_dentistry?enrichId=rgreq11db2133672c74345fd095695fd731a1- XXX&enrichSource=Y292ZXJQYWdlOzIxNTA2Mjkw OTtBUzoxMDE3NTY3MDQ5MTk1NTVAMTQwMTI 3MjA5NzI5MQ%3D%3D&el=1_x_2&_esc=publication CoverPdf
  6. Duman IG, Davul S, Gokce H, Gonenci R, Özden R, Uruc V. Effects of gaseous ozone treatment on bone regeneration in femoral defect model in rats. J Hard Tissue Biol. 2017;26:7-12.
  7. Naik SV, K R, Kohli S, Zohabhasan S, Bhatia S. OzoneA Biological Therapy in Dentistry- Reality or Myth?????. Open Dent J. 2016;10:196-206.
  8. Bocci V, Zanardi I, Travagli V. Oxygen/ozone as a medical gas mixture. A critical evaluation of the various methods clarifies positive and negative aspects. Med Gas Res. 2011;1:6.
  9. Ozdemir H, Toker H, Balcı H, Ozer H. Effect of ozone therapy on autogenous bone graft healing in calvarial defects: a histologic and histometric study in rats. J Periodont Res. 2013;48:722–6.
  10. Cade WT. Diabetes-related microvascular and macrovascular diseases in the physical therapy setting. Phys Ther. 2008;88:1322-35.
  11. Schalkwijk CG, Stehouwer CD. Vascular complications in diabetes mellitus: the role of endothelial dysfunction. Clin Sci (Lond). 2005; 109:143-59.
  12. Nanci A, Somerman M. The periodontium. In: Nanci A (ed). Ten Cate’s Oral histology: development, structure, and function. Antonio Nanci. 8th ed. Canada, St. Louis: Harcourt Health Sciences; 2013.
  13. Politis C, Schoenaers J, Jacobs R, Agbaje JO. Wound Healing Problems in the Mouth Constantinus. Front Physiol. 2016;7:507.
  14. Kanyama M, Kuboki T, Akiyama K, Nawachi K, Miyauchi FM, Yatani H, et al. Connective tissue growth factor expressed in rat alveolar bone regeneration sites after tooth extraction. Arch Oral Biol. 2003;48:723-30.
  15. Trombelli L, Farina R, Marzola A, Bozzi L, Liljenberg B, Lindhe J. Modeling and remodeling of human extraction sockets. J Clin Periodontol. 2008;35:630–9.
  16. Alan H, Vardi N, Özgür C, Acar AH, Yolcu Ü, Doğan DO. Comparison of the effects of low-level laser therapy and ozone therapy on bone healing. J Craniofac Surg. 2015;26:e396-400.
  17. Toker H, Özdemir H, Kuzu TE, Özer H. The effects of allograft combined with ozone therapy on regeneration of calvarial defects in rats. Cumhuriyet Dent J. 2016;19:205- 13.
  18. Irban A, Uslu S, Gereli A, Aydinlar EI, Karyemez PE, Luleci N, et al. The effect of ozone therapy on experimental bone fracture healing in rat. Int Res J Public Environ Health. 2015;2:159-66.
  19. Bocci V, Travagli V, Zanardi I. May oxygen-ozone therapy improves cardiovascular disorders? Cardiovasc Hematol Disord Drug Targets. 2009;9:78-85.
  20. Travagli V, Zanardi I, Valacchi G, Bocci V. Ozone and ozonated oils in skin diseases: A Review. Mediators Inflamm. 2010;2010:610418.
  21. Jiao H, Xiao E, Graves DT. Diabetes and its effect on bone and fracture healing. Curr Osteoporos Rep. 2015;13:327- 35.
  22. Cardaropoli G, Araújo M, Lindhe J. Dynamics of bone tissue formation in tooth extraction sites An experimental study in dogs. J Clin Periodontol. 2003;30:809-18.
  23. Araújo MG, Silva CO, Misawa M, Sukekava F. Alveolar socket healing: what can we learn? Periodontol 2000. 2015;68:122-34.
  24. Santana RB, Xu L, Chase HB, Amar S, Graves DT, Trackman PC. A Role for Advanced Glycation End Products in Diminished Bone Healing in Type 1 Diabetes. Diabetes. 2003;52:1502-10.
  25. Marin C, Luyten FP, Van der Schueren B, Kerckhofs G, Vandamme K. The impact of type 2 diabetes on bone fracture healing. Front Endocrinol (Lausanne). 2018;9:6.
  26. Alpan AL, Toker H, Ozer H. Ozone therapy enhances osseous healing in rats with diabetes with calvarial defects: A morphometric and immunohistochemical study. J Periodontol. 2016;87:982-9.
  27. Baraka S, Seif-El Nasr A; Abdel-Maksoud N, El-Ebiary F, Amer H, Zaghloul A, et al. The possible role of medical ozone in angiogenesis. Munich. 2003;1-2
  28. Clavo B, Pérez JL, López L, Suárez G, Lloret M, Rodríguez V, et al. Ozone therapy for tumor oxygenation: a pilot study. Evid Based Complement Alternat Med. 2004;1:93-8

Files

Share

How to cite

Statistics