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Abdelrehim, M., Mohy El Din, M., El-Shabrawy, S., Fahmy, A., Abdelhamid, S., Ramadan, H. (2019). SYNTHESIS AND CHARACTERIZATION OF METALLIC AND POLYMERIC NANOPARTICLES AND THEIR EFFECT ON THE ANTIBACTERIAL PROPERTIES OF MICROHYBRID COMPOSITE RESIN. Alexandria Dental Journal, 44(2), 39-45. doi: 10.21608/adjalexu.2019.57361
Mona M. Abdelrehim; Mona H. Mohy El Din; Sonia M. El-Shabrawy; Amal E. Fahmy; Sarah M. Abdelhamid; Heba S. Ramadan. "SYNTHESIS AND CHARACTERIZATION OF METALLIC AND POLYMERIC NANOPARTICLES AND THEIR EFFECT ON THE ANTIBACTERIAL PROPERTIES OF MICROHYBRID COMPOSITE RESIN". Alexandria Dental Journal, 44, 2, 2019, 39-45. doi: 10.21608/adjalexu.2019.57361
Abdelrehim, M., Mohy El Din, M., El-Shabrawy, S., Fahmy, A., Abdelhamid, S., Ramadan, H. (2019). 'SYNTHESIS AND CHARACTERIZATION OF METALLIC AND POLYMERIC NANOPARTICLES AND THEIR EFFECT ON THE ANTIBACTERIAL PROPERTIES OF MICROHYBRID COMPOSITE RESIN', Alexandria Dental Journal, 44(2), pp. 39-45. doi: 10.21608/adjalexu.2019.57361
Abdelrehim, M., Mohy El Din, M., El-Shabrawy, S., Fahmy, A., Abdelhamid, S., Ramadan, H. SYNTHESIS AND CHARACTERIZATION OF METALLIC AND POLYMERIC NANOPARTICLES AND THEIR EFFECT ON THE ANTIBACTERIAL PROPERTIES OF MICROHYBRID COMPOSITE RESIN. Alexandria Dental Journal, 2019; 44(2): 39-45. doi: 10.21608/adjalexu.2019.57361

SYNTHESIS AND CHARACTERIZATION OF METALLIC AND POLYMERIC NANOPARTICLES AND THEIR EFFECT ON THE ANTIBACTERIAL PROPERTIES OF MICROHYBRID COMPOSITE RESIN

Article 7, Volume 44, Issue 2, August 2019, Page 39-45  XML PDF (720.6 K)
DOI: 10.21608/adjalexu.2019.57361
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Authors
Mona M. Abdelrehim1; Mona H. Mohy El Din2; Sonia M. El-Shabrawy3; Amal E. Fahmy4; Sarah M. Abdelhamid5; Heba S. Ramadan6
1Assistant Lecturer at the Dental Biomaterials Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
2-Professor of Dental Biomaterials, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
3-Professor of Dental Biomaterials, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
4Professor of Dental Biomaterials, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
5-Assistant Professor of Microbiology, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt
6Assistant Professor of Medical Biophysics, Medical Research Institute, Alexandria University, Alexandria, Egypt
Abstract
INTRODUCTION: During the past decade, composites have become the most commonly used restorative materials. The rate of dental caries following treatment with composite resin is high. Therefore, one of the most applicable methods for preventing enamel demineralization around the restorations is using dental materials resistant to the bacterial accumulation OBJECTIVES: To synthesis and characterize antibacterial nanoparticles and to evaluate the effect of blending microhybrid composite with zinc oxide nanoparticles (ZnO), Chitosan (Cs) and combination of both Chitosan/Zinc oxide nanoparticles (Cs/ZnO) and properties of these nanoparticles on the composite resin. MATERIALS AND METHODS: Three antibacterial nanoparticles were prepared and characterized in terms of particle size, zeta potential, shape, morphology and functional group determination. Minimum inhibitory concentration of the nanoparticles was determined. The nanoparticles were incorporated into commercial microhybrid composite resin. The antibacterial properties against Streptococcus mutans were evaluated by disc diffusion test and direct contact test. The results were analyzed using ANOVA test at p ≤ .05 significance level. RESULTS: For agar diffusion disc, incorporation of ZnO nanoparticles into the composite resin results in an antibacterial effect which lasted for up to 12 weeks, while for the Cs and Cs/ZnO nanoparticles the antibacterial effect lasted for up to 2 weeks. The direct contact test visualized under SEM also showed that incorporation of ZnO nanoparticles into composite resin to be the most inhibitory in all the 4 groups, denoting that ZnO-NPs has a far better inhibitory effect than Cs-NPs and Cs/ ZnO-NPs. CONCLUSIONS Antibacterial nanoparticles could be synthesized and characterized by Zetasizer NanoZS, scanning electron, transmission microscopy and Fourier transform infrared spectroscopy. Incorporation of ZnO, Cs and Cs/ZnO nanoparticles into the composite resin could significantly inhibit the S. mutans. The antimicrobial efficacy of the ZnO nanoparticles blended with microhybrid composite resin was confirmed for a duration up to 12 weeks
Keywords
composite resin; ZnO; Chitosan nanoparticles
Main Subjects
Dental biomaterials
References
  1. Delaviz Y, Finer Y, Santerre JP. Biodegradation of resin composites and adhesives by oral bacteria and saliva: A rationale for new material designs that consider the clinical environment and treatment challenges. Dent Mater. 2014;30:16–32.
  2. Nedeljkovic I, Teughels W, De Munck J, Van Meerbeek B, Van Landuyt KL. Is secondary caries with composites a material-based problem? Dent Mater. 2015;31:e247–77.
  3. Sevinç BA, Hanley L. Antibacterial activity of dental composites containing zinc oxide nanoparticles. J Biomed Mater. 2010;94:22–31.
  4. Tavassoli Hojati S, Alaghemand H, Hamze F, Ahmadian Babaki F, Rajab-Nia R, Rezvani MB, et al. Antibacterial, physical and mechanical properties of flowable resin composites containing zinc oxide nanoparticles. Dent Mater. 2013;29:495–505.
  5. Mirhashemi AH, Bahador A, Kassaee MZ, Daryakenari G, Sadegh M, Akhoundi A, et al. Antimicrobial Effect of Nano-Zinc Oxide and Nano-Chitosan Particles in Dental Composite Used in Orthodontics. J Med Bacteriol. 2013;2:1–10.
  6. Javidi M, Zarei M, Naghavi N, Mortazavi M, Nejat AH. Zinc oxide nano-particles as sealer in endodontics and its sealing ability. Contemp Clin Dent. 2014;5:35–9.
  7. Martinez-Gutierrez F, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, et al. Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomedicine Nanotechnology, Biol Med. 2010;6:681–8.
  8. Hernández-Sierra JF, Ruiz F, Cruz Pena DC, MartínezGutiérrez F, Martínez AE, de Jesús Pozos Guillén A, et al. The antimicrobial sensitivity of Streptococcus mutans to nanoparticles of silver, zinc oxide, and gold. Nanomedicine Nanotechnology, Biol Med. 2008;4:237–40.
  9. Kim J-S, Shin D-H. Inhibitory effect on Streptococcus mutans and mechanical properties of the chitosan containing composite resin. Restor Dent Endod. 2013;38:36–42.
  10. Qi L, Xu Z, Jiang X, Hu C, Zou X. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res. 2004;339:2693–700.
  11. AbdElhady MM. Preparation and Characterization of Chitosan/Zinc Oxide Nanoparticles for Imparting Antimicrobial and UV Protection to Cotton Fabric. Int J Carbohydr Chem. 2012;2012:1–6.
  12. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, 3rd ed. CLSI document M31-A3. Clinical and Laboratory Standards. 2015.
  13. Mirani SA, Sangi L, Kumar N, Ali D. Investigating the antibacterial effect of chitosan in dental resin composites: A pilot study. Pak Oral Dental J. 2015;35:15–8.
  14. Kasraei S, Sami L, Hendi S, Alikhani M-Y, Rezaei-Soufi L, Khamverdi Z. Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus. Restor Dent Endod. 2014;39:109–14.
  15. Mahapoka E, Arirachakaran P, Watthanaphanit A, Rujiravanit R, Poolthong S. Chitosan whiskers from shrimp shells incorporated into dimethacrylate-based dental resin sealant. Dent Mater J. 2012;31:273–9.
  16. Heshmat H, Hajian M, Ganjkar MH, Arjomand ME. Effect of Tea on Color Change of Silorane and Methacrylate based Composite Resins. J Islam Dent Assoc Iran. 2013;25:142– 6.
  17. Melo MAS, Guedes SFF, Xu HHK, Rodrigues LKA. Nanotechnology-based restorative materials for dental caries management. Trends Biotechnol. 2013;31:459–67.
  18. Memarzadeh K, Sharili AS, Huang J, Rawlinson SCF, Allaker RP. Nanoparticulate zinc oxide as a coating material for orthopedic and dental implants. J Biomed Mater Res Part A. 2015;103:981–9.
  19. Niu LN, Fang M, Jiao K, Tang LH, Xiao YH, Shen LJ, et al. Tetrapod-like zinc oxide whisker enhancement of resin composite. J Dent Res. 2010;89:746–50.
  20. Sirelkhatim A, Mahmud S, Seeni A, Kaus NHM, Ann LC, Bakhori SKM, et al. Review on zinc oxide nanoparticles: Antibacterial activity and toxicity mechanism. Nano-Micro Lett. 2015;7:219–42.
  21. Hajipour MJ, Fromm KM, Ashkarran AA, de Aberasturi DJ, de Larramendi IR, Rojo T, et al. Antibacterial properties of nanoparticles. Trends Biotechnol. 2012;30:499–511.
  22. Giertsen E, Scheie AA, Rölla G. Inhibition of plaque formation and plaque acidogenicity by zinc and chlorhexidine combinations. Eur J Oral Sci. 1988;96:541– 50.
  23. Friedman AJ, Phan J, Schairer DO, Champer J, Qin M, Pirouz A, et al. Antimicrobial and anti-inflammatory activity of chitosan--alginate nanoparticles: A targeted therapy for cutaneous pathogens. J Invest Dermatol. 2013;133:1231–9.
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