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El Shafie, S., El Shimy, A., El Sheredy, A., EL. Mousafa, M. (2019). ANTIFUNGAL EFFECT OF PHOTOCATALYTIC NANO -TITANIUM DIOXIDE INCORPORATED IN SILICONE ELASTOMER:. Alexandria Dental Journal, 44(2), 52-60. doi: 10.21608/adjalexu.2019.57363
Sarah Y. El Shafie; Ahmed M. El Shimy; Amel G. El Sheredy; Maisa EL. Mousafa. "ANTIFUNGAL EFFECT OF PHOTOCATALYTIC NANO -TITANIUM DIOXIDE INCORPORATED IN SILICONE ELASTOMER:". Alexandria Dental Journal, 44, 2, 2019, 52-60. doi: 10.21608/adjalexu.2019.57363
El Shafie, S., El Shimy, A., El Sheredy, A., EL. Mousafa, M. (2019). 'ANTIFUNGAL EFFECT OF PHOTOCATALYTIC NANO -TITANIUM DIOXIDE INCORPORATED IN SILICONE ELASTOMER:', Alexandria Dental Journal, 44(2), pp. 52-60. doi: 10.21608/adjalexu.2019.57363
El Shafie, S., El Shimy, A., El Sheredy, A., EL. Mousafa, M. ANTIFUNGAL EFFECT OF PHOTOCATALYTIC NANO -TITANIUM DIOXIDE INCORPORATED IN SILICONE ELASTOMER:. Alexandria Dental Journal, 2019; 44(2): 52-60. doi: 10.21608/adjalexu.2019.57363

ANTIFUNGAL EFFECT OF PHOTOCATALYTIC NANO -TITANIUM DIOXIDE INCORPORATED IN SILICONE ELASTOMER:

Article 9, Volume 44, Issue 2, August 2019, Page 52-60  XML PDF (504.3 K)
DOI: 10.21608/adjalexu.2019.57363
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Authors
Sarah Y. El Shafie1; Ahmed M. El Shimy2; Amel G. El Sheredy3; Maisa EL. Mousafa4
1Bachelor of Dentistry, Removable Prosthodontics Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
2Professor of Removable Prosthodontics, Head of Removable Prosthodontics Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
3Assistant Professor of Microbiology, Microbiology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
4Lecturer of Medical Biophysics, Medical Biophysics Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Abstract
INTRODUCTION: Titanium dioxide nanoparticles may improve the antifungal properties of silicone elastomer prosthetic materials. OBJECTIVES: To evaluate the photocatalytic antifungal activity of silicone elastomer incorporated with titanium dioxide nanoparticles against Candida albicans. MATERIALS AND METHODS Titanium dioxide nano-particles (Aeroxide® TiO2 P25, Sigma Aldrich, USA) were characterized using xray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), uv-vis spectrophotometeric analysis, methylene blue degradation efficiency test and direct contact test. Sixty six disc shaped specimens (6 mm diameter x 1mm thickness) of silicone elastomer (SE) (MED-4210, Factor II, USA) were included and divided into 6 groups based on the concentration of nano-TiO2 in silicone elastomer (0%, 2%, 4%, 6%, 8%, 10%) (W/W). Impregnated culture test was used to evaluate the antifungal activity of all SE samples against candida albicans as colonies forming units (CFUs) of treated aqueous suspension of candida albicans were measured at 0, 30, 60, 90 and 120 min follow‑up under UVA light. The dispersion of TiO2 within SE matrix was analysed by Laser scanning microscopy (LSM). RESULTS: Data were analysed using ANOVA test and Tuckey's Post Hoc test. Nano-TiO2 characterization tests confirmed the crystalline structure (85% anatase and 15% rutile), shape (irregular faceted), size (22nm), absorption spectrum (320-400nm) and the photocatalytic antifungal ability of the as-purchased Aeroxide TiO2 P25. Planktonic cultures of candida albicans exposed to SE/TiO2 samples reduced candida albicans count by 4% - 99.99%, depending on the nanoparticles concentration and follow-up time. The candida albicans counts did not decrease in group I without TiO2. LSM of SE specimens showed finer dispersion of TiO2 within SE matrix in group II, III and IV than that in group V and VI. CONCLUSIONS: Incorporation of TiO2 P25 within SE (MED-4210) shows promising inhibitory activity against candida albicans.
Keywords
antifungal activity; Candida albicans; photocatalytic nano-titanium dioxide; silicone elastomer
Main Subjects
Prosthodontics
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