THE EFFECT OF DEPROTEINIZATION PRIOR TO ENAMEL ACID ETCHING ON PIT AND FISSURE SEALANT SHEAR BOND STRENGTH AND NANOLEAKAGE IN PERMANENT TEETH (IN-VITRO STUDY)

Document Type : Original Article

Authors

1 Bachelor of Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.

2 Professor of Pediatric Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.

3 Professor of Dental Biomaterials department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt

4 Associate Professor of Pediatric Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.

Abstract

INTRODUCTION: Enamel deproteinization before acid etching can be used as a method to improve and enhance the sealant’s retentive and sealing ability OBJECTIVES: To assess and compare the sealant shear bond strength and nanoleakage after placement on etched enamel with and without prior deproteinization. MATERIALS AND METHODS: Eighty extracted sound human permanent teeth were selected. These were randomly divided into two groups according to the enamel treatment methods, where group A (experimental group): deproteinization prior to acid etching and group B (control group): acid etching without deproteinization. Each group was further subdivided equally into two subgroups (n=20) according to the test used whether shear bond strength and nanoleakage respectively. Shear bond strength test: Shear bond strength was measured using universal testing machine. Mode of failure of each specimen was determined using stereomicroscope. Nanoleakage test: Sealed teeth were then thermocycled, immersed in 50% silver nitrate solution for 24 hours, sectioned and evaluated quantitatively using energy dispersive x-ray spectrometer (EDX) and qualitatively using scanning electron microscope (SEM). Data were collected, tabulated and statistically analyzed using Mann-Whitney U test (P<0.05), chi square test and Post-hoc analysis. RESULTS: The rate of sealant shear bond strength was similar between the two groups (P=0.678), but the rate of sealant nanoleakage was significantly lower in the enamel deproteinization group (P<0.001) than in the control group. CONCLUSIONS: Deproteinization method prior to enamel acid etching improved shear bond strength and sealing ability of sealants..

Keywords

Main Subjects

References

  1. Nardi GM, Mazur M, Corridore D, Capocci M, Rinaldo FM, Maruotti A et al. Clinical comparison between an opaque and a clear pit and fissures sealants: a 12-month split-mouth, randomized controlled study. Clin Ther. 2018;169:e5-e9.
  2. Agarwal A, Shigli A. Comparison of six different methods of cleaning and preparing occlusal fissure surface before placement of pit and fissure sealant: An in vitro study. J Indian Soc Pedod Prev Dent. 2012;30:51-5.
  3. Bravo M, Montero J, Bravo JJ, Baca P, Llodra JC. Sealant and fluoride varnish in caries: a randomized trial. J Dent Res. 2005;4:1138-43.
  4. Borsatto MC, Corona SA, Dibb RG, Ramos RP, Pécora JD. Microleakage of a resin sealant after acid-etching, Er:YAG laser irradiation and air-abrasion of pits and fissures. J Clin Laser Med Surg. 2001;19:83-7.
  5. Sano H, Takatsu T, Ciucchi B, Horner JA, Matthews WG, Pashley DH. Nanoleakage: leakage within the hybrid layer. Oper Dent. 1995;20:18-25.
  6. Buonocore MG. A simple method of increasing the adhesion of acrylic filling materials to enamel surfaces. J Dent Res. 1955;34:849-53.
  7. Harleen N, Ramakrishna Y, Munshi AK. Enamel deproteinization before acid etching and its effect on the shear bond strength - an in vitro study. J Clin Pediatr Dent. 2011;36:19-23.
  8. Espinosa R, Valencia R, Uribe M, Ceja I, Saadia M. Enamel deproteneization and its effect on acid etching: An in vitro study. J Clin Pediatr Dent. 2008;33:13-20.
  9. Hobson RS, Crotty T, Thomason JM, Jepson NJ. A quantitative study of enamel acid etch patterns on surfaces used for retention of resin- bonded fixed prostheses. Eur J Prosthodont Restor Dent. 2005;13:123- 8.
  10. Abdelmegid FY. Effect of deproteinization before and after acid etching on the surface roughness of immature permanent enamel. Niger J Clin Pract. 2018;21:591-6
  11. Garrocho-Rangel A, Lozano-Vázquez C, Butrón-TellezGirón C, Escobar-García D, Ruíz-Rodriguez S, PozosGuillén A. In vitro assessment of retention and microleakage in pit and fissure sealants following enamel pre-etching with sodium hypochlorite deproteinisation. Eur J Paediatr Dent. 2015;16:212-6.
  12. Bhoomika A, Ramakrishna Y, Baliga MS, Munshi AK. Enamel deproteinization before acid etching – a Scanning electron microscopic observation. J Clin Pediatr Dent. 2010;35:169-72.
  13. Ramakrishna Y, Bhoorsika A, Harleen N, Munshi AK. Enamel deproteneization after acid etching – Is it worth the effort? Dentistry. 2014;4:200-4.
  14. Hatibovic-Kofman S, Butler SA, Sadek H. Microleakage of three sealents followed by Conventional, bur and air abrasion preparation of pit and fissure. Int J Peadiatr Dent. 2001;11:409-16.
  15. Schulz KF, Grimes DA. Generation of allocation sequences in randomised trials: chance, not choice. Lancet. 2002;359:515-9.
  16. Margvelashvili M, Vichi A, Carrabba M, Goracci C, Ferrari M. Bond strength to unground enamel and sealing ability in pits and fissures of a new self-adhering flowable resin composite. J Clin Pediatr Dent. 2013;37:397-402.
  17. Ellis RW, Latta MA, Westerman GH. Effect of air abrasion and acid etching on sealant retention: an in vitro study. Pediatr Dent. 1999;21:316-9.
  18. Sharaf A. The use of a self-etching adhesive in the application of pit and fissure sealant: an in vivo and in vitro study. Egy Dent J. 2003;49:1319-26.
  19. Peutzfeldt A, Nielsen LA. Bond strength of a sealant to primary and permanent enamel: phosphoric acid versus self-etching adhesive. Pediatr Dent. 2004;26:240-4.
  20. Pardi V, Sinhoreti MA, Pereira AC, Ambrosano GM, Meneghim Mde C. In vitro evaluation of microleakage of different materials used as pit and fissure sealants. Braz Dent J. 2006;17:49-52.
  21. Tay FR, Pashley DH, Yoshiyama M. Two modes of nanoleakage expression in single-step adhesives. J Dent Res. 2002;81:472-6.
  22. De Carvalho Filho AC, Sanches RP, Martin AA, Do Espírito Santo AM, Soares LE. Energy dispersive X-ray spectrometry study of the protective effects of fluoride and varnish and gel on enamel erosion. Microscc Res Tech. 2011;74:839-44.
  23. El-Badrawy W, Hafez RM, Abo El Naga AI, Ahmed DR. Nanoleakage for Self-Adhesive Resin Cements used in Bonding CAD/CAD Ceramic Material to Dentin. Eur J Dent. 2011;5:281-90.
  24. Atwan SM, Sullivan RE. In vitro evaluation of the tensile bond strength of a chemically initiated and a visibly initiated sealant with SEM observations. Pediatr Dent. 1987;9:147-51.
  25. IBM Corp. IBM SPSS Statistics for Windows, Version 21.0. Armonk, NY: IBM Corp.; Released 2012.
  26. Gandhi S, Crawford P, Shellis P. The use of a ‘bleach – etch – seal’ deproteinization technique on MIH affected enamel. Int J Pediatr Dent. 2012;22:427- 34.
  27. Espinosa R, Valencia R, Uribe M, Ceja I, Cruz J, Saadia M. Resin Replica in enamel deproteinization and its effect on acid etching. J Clin Pediatr Dent. 2010;35:47-52.
  28. Fouad W, El Motayam KM, Youssef R. Assessment and Comparison of Nanoleakage and Resin Tag Length of Three Different Pit and Fissure Sealants: An In-Vitro Scanning Electron Microscope Study. J Am Sci. 2013;9:329-37.
  29. Prieto LT, Souza-Júnior EJ, Araújo CT, Lima AF, Dias CT, Paulillo LA. Nanoleakage evaluation of resin luting systems to dental enamel and leucite-reinforced ceramic. Microsc Res Tech. 2012;75:671-6.
  30. Yavuz I, Aydin AH, Ulku R, Dulgergil TC, Akdag MZ. New technique: Measurement of microleakage volume in the marginal gaps of the dental restorations. Biotechnol Biotech Eq. 2005;19:184-91.
  31. Naaman R, El-Housseiny AA, Alamoudi N. The Use of Pit and Fissure Sealants—A Literature Review. Dent J (Basel). 2017;5:pii:E34.
  32. Babaji P, Vaid S, Deep S, Mishra S, Srivastava M, Manjooran T. In vitro evaluation of shear bond strength and microleakage of different pit and fissure sealants. J Int Soc Prev Community Dent. 2016;6:S111-5
Alexandria Dental Journal
Volume 44, Issue 2
August 2019
Pages 108-113

Files

Share

How to cite

Statistics