Mowiena, O., Wahba, N., El-Shabrawy, S., Talaat, D. (2019). THE EFFECT OF DEPROTEINIZATION PRIOR TO ENAMEL ACID ETCHING ON PIT AND FISSURE SEALANT SHEAR BOND STRENGTH AND NANOLEAKAGE IN PERMANENT TEETH (IN-VITRO STUDY). Alexandria Dental Journal, 44(2), 108-113. doi: 10.21608/adjalexu.2019.57373
Omneya M. Mowiena; Nadia A. Wahba; Sonia El-Shabrawy; Dalia M. Talaat. "THE EFFECT OF DEPROTEINIZATION PRIOR TO ENAMEL ACID ETCHING ON PIT AND FISSURE SEALANT SHEAR BOND STRENGTH AND NANOLEAKAGE IN PERMANENT TEETH (IN-VITRO STUDY)". Alexandria Dental Journal, 44, 2, 2019, 108-113. doi: 10.21608/adjalexu.2019.57373
Mowiena, O., Wahba, N., El-Shabrawy, S., Talaat, D. (2019). 'THE EFFECT OF DEPROTEINIZATION PRIOR TO ENAMEL ACID ETCHING ON PIT AND FISSURE SEALANT SHEAR BOND STRENGTH AND NANOLEAKAGE IN PERMANENT TEETH (IN-VITRO STUDY)', Alexandria Dental Journal, 44(2), pp. 108-113. doi: 10.21608/adjalexu.2019.57373
Mowiena, O., Wahba, N., El-Shabrawy, S., Talaat, D. THE EFFECT OF DEPROTEINIZATION PRIOR TO ENAMEL ACID ETCHING ON PIT AND FISSURE SEALANT SHEAR BOND STRENGTH AND NANOLEAKAGE IN PERMANENT TEETH (IN-VITRO STUDY). Alexandria Dental Journal, 2019; 44(2): 108-113. doi: 10.21608/adjalexu.2019.57373
THE EFFECT OF DEPROTEINIZATION PRIOR TO ENAMEL ACID ETCHING ON PIT AND FISSURE SEALANT SHEAR BOND STRENGTH AND NANOLEAKAGE IN PERMANENT TEETH (IN-VITRO STUDY)
1Bachelor of Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
2Professor of Pediatric Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
3Professor of Dental Biomaterials department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
4Associate Professor of Pediatric Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Abstract
INTRODUCTION: Enamel deproteinization before acid etching can be used as a method to improve and enhance the sealant’s retentive and sealing ability OBJECTIVES: To assess and compare the sealant shear bond strength and nanoleakage after placement on etched enamel with and without prior deproteinization. MATERIALS AND METHODS: Eighty extracted sound human permanent teeth were selected. These were randomly divided into two groups according to the enamel treatment methods, where group A (experimental group): deproteinization prior to acid etching and group B (control group): acid etching without deproteinization. Each group was further subdivided equally into two subgroups (n=20) according to the test used whether shear bond strength and nanoleakage respectively. Shear bond strength test: Shear bond strength was measured using universal testing machine. Mode of failure of each specimen was determined using stereomicroscope. Nanoleakage test: Sealed teeth were then thermocycled, immersed in 50% silver nitrate solution for 24 hours, sectioned and evaluated quantitatively using energy dispersive x-ray spectrometer (EDX) and qualitatively using scanning electron microscope (SEM). Data were collected, tabulated and statistically analyzed using Mann-Whitney U test (P<0.05), chi square test and Post-hoc analysis. RESULTS: The rate of sealant shear bond strength was similar between the two groups (P=0.678), but the rate of sealant nanoleakage was significantly lower in the enamel deproteinization group (P<0.001) than in the control group. CONCLUSIONS: Deproteinization method prior to enamel acid etching improved shear bond strength and sealing ability of sealants..
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