EFFECT OF AUTOGENOUS DENTIN GRAFT COMBINED WITH PLATELET RICH PLASMA ON ALVEOLAR BONE HEALING AFTER TOOTH EXTRACTION IN RABBIT

Document Type : Original Article

Authors

1 Demonstrator of Oral Biology -Faculty of Dentistry –Alexandria University

2 Professor of Oral Biology -Faculty of Dentistry –Alexandria University

3 Professor of Oral and Maxillofacial Surgery - Faculty of Dentistry - Alexandria University

Abstract

INTRODUCTION: Alveolar ridge preservation strategies are indicated to minimize the loss of ridge volume that typically follows tooth extraction. Dentin and bone are mineralized tissues and almost similar in chemical components. Particulate dentin has a plenty of growth factors such as bone morphogenetic proteins (BMP) that can induce new bone formation. Platelets play a fundamental role in hemostasis and are a natural source of growth factors. Mixture of particulate dentin and platelet-rich plasma can have an osteoinductive effect during different stages of bone healing. OBJECTIVES: To investigate the biological effect of autogenous dentin graft combined with platelet rich plasma on alveolar bone healing following tooth extraction in rabbit. MATERIALS AND METHODS: Seven healthy male New Zealand rabbits weighing 3kg (±250 g) were included in this study. The upper right incisor of each rabbit was extracted .The root of each incisor was used as a source of dentin. Root dentin was ground into powder by means of mortar and pestle.2 ml blood were taken from each rabbit. Platelet rich plasma was separated from other blood components by centrifuge machine. The left and right lower first premolars were extracted at the same time .The sockets of the lower right first premolars were filled by the mixture of dentin and platelet rich plasma (study group) while sockets of the lower left premolars were left to heal spontaneously (control group). Comparison of the healing features between the two groups was made histologically and histomorhometrically after 6 weeks. RESULTS: New bone formation was noticed in the sockets loaded with dentin and platelet rich plasma mixture. The newly formed bone was significantly higher when compared to that in the control group. CONCLUSIONS combination of dentin and platelet rich plasma induces new bone formation.

Keywords

References

  1. Araújo MG, Lindhe J. Dimensional ridge alterations following tooth extraction. An experimental study in the dog. Journal of clinical periodontology. 2005;32(2):212-8.
  2. Fickl S, Zuhr O, Wachtel H, Stappert CF, Stein JM, Hürzeler MB. Dimensional changes of the alveolar ridge contour after different socket preservation techniques. Journal of clinical periodontology. 2008;35(10):906-13.
  3. Park S-M, Um I-W, Kim Y-K, Kim K-W. Clinical application of auto-tooth bone graft material. Journal of the Korean Association of Oral and Maxillofacial Surgeons. 2012;38(1):2-8.
  4. Bessho K, Tagawa T, Murata M. Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction. Journal of Oral and Maxillofacial Surgery. 1990;48(2):162-9.
  5. Becker W, Becker BE, Caffesse R. A comparison of demineralized freeze-dried bone and autologous bone to induce bone formation in human extraction sockets. Journal of periodontology. 1994;65(12):1128-33.
  6. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 1998;85(6):638-46.
  7. Fennis J, Stoelinga P, Jansen J. Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate cortico-cancellous bone grafts and platelet rich plasma in goats. International journal of oral and maxillofacial surgery. 2004;33(1):48- 55.
  8. Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plastic and reconstructive surgery. 2004;114(6):1502-8.
  9. Kasten P, Vogel J, Geiger F, Niemeyer P, Luginbühl R, Szalay K. The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials. 2008;29(29):3983-92.
  10. Lekovic V, Camargo PM, Weinlaender M, Vasilic N, Kenney EB. Comparison of platelet-rich plasma, bovine porous bone mineral, and guided tissue regeneration versus platelet-rich plasma and bovine porous bone mineral in the treatment of intrabony defects: a reentry study. Journal of periodontology. 2002;73(2):198-205.
  11. Dallari D, Fini M, Stagni C, Torricelli P, Nicoli Aldini N, Giavaresi G, et al. In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐ rich plasma, and freeze‐dried bone allografts, alone and in combination. Journal of Orthopaedic Research. 2006;24(5):877-88.
  12. Su-Gwan Kim D, Chae-Heon Chung D, Young-Kyun Kim D, Joo-Cheol Park D, Sung-Chul Lim M. The use of particulate dentin-plaster of Paris combination with/without platelet-rich plasma in the treatment of bone defects around implants. The International journal of oral & maxillofacial implants. 2002:86-94.
  13. Everts PA, Brown Mahoney C, Hoffmann JJ, Schönberger JP, Box HA, Van Zundert A, et al. Platelet-rich plasma preparation using three devices: implications for platelet activation and platelet growth factor release. Growth Factors. 2006;24(3):165-71.
  14. Yugoshi LI, Sala MA, Brentegani LG, Carvalho TLL. Histometric study of socket healing after tooth extraction in rats treated with diclofenac. Brazilian dental journal. 2002;13(2):92-6.
  15. Hsu H, Lachenbruch PA. Paired t test. Wiley Encyclopedia of Clinical Trials. 2008.
  16. Kim Y-K, Kim S-G, Byeon J-H, Lee H-J, Um I-U, Lim SC, et al. Development of a novel bone grafting material using autogenous teeth. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2010;109(4):496-503.
  17. Wang H-L, Avila G. Platelet rich plasma: myth or reality? European journal of dentistry. 2007;1(4):192.
  18. Freymiller EG, Aghaloo TL. Platelet-rich plasma: ready or not? Journal of Oral and Maxillofacial Surgery. 2004;62(4):484-8.
  19. Jakse N, Tangl S, Gilli R, Berghold A, Lorenzoni M, Eskici A, et al. Influence of PRP on autogenous sinus grafts. Clinical Oral Implants Research. 2003;14(5):578- 83.
  20. Murata M. Bone engineering using human demineralized dentin matrix and recombinant human BMP-2. Journal of Hard Tissue Biology. 2005;14(2):80-1.
  21. Ike M, Urist MR. Recycled dentin root matrix for a carrier of recombinant human bone morphogenetic protein. Journal of Oral Implantology. 1998;24(3):124-32.
  22. Murata M, Hino J, Ito K. Biochemical and histomorphometrical analyses of bone and cartilage induced by human decalcified dentin matrix and BMP-2. 구강생물학연구. 2011;35(1):9-14.
  23. Gomes MF, de Freitas Banzi ÉC, De Souza Setúbal Destro MF, Lavinicki V, Goulart MdGV. Homogenous demineralized dentin matrix for application in cranioplasty of rabbits with alloxan-induced diabetes: histomorphometric analysis. International Journal of Oral & Maxillofacial Implants. 2007;22(6).
  24. Kim Y-K, Lee J, Um I-W, Kim K-W, Murata M, Akazawa T, et al. Tooth-derived bone graft material. Journal of the Korean Association of Oral and Maxillofacial Surgeons. 2013;39(3):103-11.
  25. Kim Y-K, Kim S-G, Yun P-Y, Yeo I-S, Jin S-C, Oh J-S, et al. Autogenous teeth used for bone grafting: a comparison with traditional grafting materials. Oral surgery, oral medicine, oral pathology and oral radiology. 2014;117(1):e39-e45.
  26. Simon D, Manuel S, Maneul S, Geetha V, Naik B. Potential for osseous regeneration of platelet-rich plasma-- a comparative study in mandibular third molar sockets. Indian journal of dental research: official publication of Indian Society for Dental Research. 2003;15(4):133-6.
  27. Albanese A, Licata ME, Polizzi B, Campisi G. Plateletrich plasma (PRP) in dental and oral surgery: from the wound healing to bone regeneration. Immunity & Ageing. 2013;10(1):23.
  28. Whitman DH, Berry RL, Green DM. Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. Journal of Oral and Maxillofacial Surgery. 1997;55(11):1294-9.

Files

Share

How to cite

Statistics