EXPRESSION OF Ki-67 AND CD105 MARKERS IN ORAL SQUAMOUS CELL CARCINOMA

Document Type : Original Article

Authors

1 B.D.S 2009

2 Professor of Oral Pathology, Faculty of Dentistry, Alexandria University

3 Professor in Cranio Maxillofacial and Plastic Surgery, faculty of dentistry, Alexandria University

Abstract

INTRODUCTION: Oral squamous cell carcinoma (OSCC) is the commonest malignancy in the oral cavity. It is influenced by genetic modifications; as well as numerous environmental factors, such as smoking, tobacco chewing, alcohol consumption, infection by oncogenic viruses, and low intake of fruits and vegetables. CD105 has been introduced as a marker for angiogenesis which stains vessels that are in the proliferating stage. Ki-67is a nuclear protein that is recognized as a nuclear antigen present in proliferating cells, but absent in resting cells. OBJECTIVES: To evaluate and correlate the expression of Ki-67 and CD105 in different histopathological grades of OSCC. MATERIALS AND METHODS: Immunohistochemical (IHC) study using the Ki-67 and CD105 antibody was done on 20 surgical specimens and 5 normal mucosal tissues taken from OSCC patients and healthy individuals respectively. RESULTS: Remarkable improvement in the histology and the ultrastructure of the alveolar bone of rats in Group III was observed. Moreover, hematological values revealed significant decrease in the inflammatory condition of rats with induced RA after fish oil treatment. CONCLUSIONS: Ki-67 and CD105 are expressed in OSCC biopsies. Additionally, the fraction of Ki-67positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of OSCC. CD105 microvessel density can be a useful factor for predicting the course of oral squamous cell carcinoma

Keywords

Main Subjects

References

  1. Katakura A, Yamamoto N, Sakuma T, Sugahara K, Onda T, Noguchi S, et al. A screening test for oral cancer using saliva samples: Proteomic analysis of biomarkers in whole saliva. J Oral Maxillofac Surg Med Pathol. 2013;27:1-5.
  2. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA Cancer J Clin. 2016;66:7-30.
  3. Kervancioglu E, Kosan M, Erinanc H, Gonulalan U, Oguzulgen AI, Coskun EZ, et al. Predictive values of vascular endothelial growth factor and microvessel-density levels in initial biopsy for prostate cancer. Kaohsiung J Med Sci. 2016;32:74-9.
  4. Kulasinghe A, Perry C, Warkiani ME, Blick T, Davies A, O'Byrne K, et al. Short term ex-vivo expansion of circulating head and neck tumour cells. Oncotarget. 2016;7:60101-9.
  5. de Moraes M MMC, de Almeida Freitas R, Galvão HC. Cell proliferation markers in oral squamous cell carcinoma. J Mol Biomark Diagn. 2012;S2.
  6. Zhu Y, Yang QC, Liu HB, Liu MH, Shen Y, Zhang XJ. [Expression and significance of hypoxia-inducible factor 1alpha protein and microvessel density marked by CD105 in cervical carcinoma]. Zhonghua Bing Li Xue Za Zhi. 2008;37:264-5.
  7. Tumuluri V, Thomas GA, Fraser IS. Analysis of the Ki-67 antigen at the invasive tumour front of human oral squamous cell carcinoma. J Oral Pathol Med. 2002;31:598- 604.
  8. Kurokawa H, Zhang M, Matsumoto S, Yamashita Y, Tanaka T, Tomoyose T, et al. The relationship of the histologic grade at the deep invasive front and the expression of Ki-67 antigen and p53 protein in oral squamous cell carcinoma. J Oral Pathol Med. 2005;34:602- 7.
  9. Acharya S, Sivakumar AT, Shetty S. Cervical lymph node metastasis in oral squamous cell carcinoma: a correlative study between histopathological malignancy grading and lymph node metastasis. Indian J Dent Res. 2013;24:599- 604.
  10. Girardi FM, Zanella VG, Kroef RG. Correlation between clinical and pathological data and surgical margins in patients with squamous cell carcinoma of the oral cavity. Braz J Otorhinolaryngol. 2013;79:190-5.
  11. Jerjes W, Upile T, Petrie A, Riskalla A, Hamdoon Z, Vourvachis M, et al. Clinicopathological parameters, recurrence, locoregional and distant metastasis in 115 T1- T2 oral squamous cell carcinoma patients. Head Neck Oncol. 2010;2:9.
  12. Durr ML, van Zante A, Li D, Kezirian EJ, Wang SJ. Oral tongue squamous cell carcinoma in never-smokers: analysis of clinicopathologic characteristics and survival. Otolaryngol Head Neck Surg. 2013;149:89-96.
  13. Li R, Koch WM, Fakhry C, Gourin CG. Distinct epidemiologic characteristics of oral tongue cancer patients. Otolaryngol Head Neck Surg. 2013;148:792-6.
  14. Pires FR, Ramos AB, Oliveira JB, Tavares AS, Luz PS, Santos TC. Oral squamous cell carcinoma: clinicopathological features from 346 cases from a single oral pathology service during an 8-year period. J Appl Oral Sci. 2013;21:460-7.
  15. Warnakulasuriya S. Global epidemiology of oral and oropharyngeal cancer. Oral Oncology. 2009;45:309-16.
  16. Bello IO, Soini Y, Salo T. Prognostic evaluation of oral tongue cancer: means, markers and perspectives (I). Oral Oncol. 2010;46:630-5.
  17. Warnakulasuriya S. Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 2009;45:309-16.
  18. Chang TS, Chang CM, Ho HC, Su YC, Chen LF, Chou P, et al. Impact of young age on the prognosis for oral cancer: a population-based study in Taiwan. PLoS One. 2013;8:e75855.
  19. Krishna A, Singh RK, Singh S, Verma P, Pal US, Tiwari S. Demographic risk factors, affected anatomical sites and clinicopathological profile for oral squamous cell carcinoma in a north Indian population. Asian Pac J Cancer Prev. 2014;15:6755-60.
  20. Kanke M, Fujii M, Kameyama K, Kanzaki J, Tokumaru Y, Imanishi Y, et al. Role of CD44 variant exon 6 in invasion of head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 2000;126:1217-23.
  21. Chen YK, Huang HC, Lin LM, Lin CC. Primary oral squamous cell carcinoma: an analysis of 703 cases in southern Taiwan. Oral Oncol. 1999;35:173-9.
  22. Tadbir AA, Ashraf MJ, Moradi M. Clinicopathological Significance of CD105 Expression in Squamous Cell Carcinoma of the Oral Cavity. Middle East J Cancer. 2014;5:7-12.
  23. Chien CY, Su CY, Hwang CF, Chuang HC, Hsiao YC, Wu SL, et al. Clinicopathologic significance of CD105 expression in squamous cell carcinoma of the hypopharynx. Head Neck. 2006;28:441-6.
  24. Olimid DA, Simionescu CE, Margaritescu C, Florescu A. Immunoexpression of Ki67 and cyclin D1 in oral squamous carcinomas. Rom J Morphol Embryol. 2012;53(3 Suppl):795-8.
  25. Margaritescu C, Pirici D, Stinga A, Simionescu C, Raica M, Mogoanta L, et al. VEGF expression and angiogenesis in oral squamous cell carcinoma: an immunohistochemical and morphometric study. Clin Exp Med. 2010;10:209-14.
  26. Gerdes J, Schwab U, Lemke H, Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983;31:13-20.
  27. Torres-Rendon A, Roy S, Craig GT, Speight PM. Expression of Mcm2, geminin and Ki67 in normal oral mucosa, oral epithelial dysplasias and their corresponding squamous-cell carcinomas. Br J Cancer. 2009;100:1128-34.
  28. Dwivedi N, Chandra S, Kashyap B, Raj V, Agarwal A. Suprabasal expression of Ki-67 as a marker for the severity of oral epithelial dysplasia and oral squamous cell carcinoma. Contemp Clin Dent. 2013;4:7-12.
  29. Blank S, Deck C, Dreikhausen L, Weichert W, Giese N, Falk C, et al. Angiogenic and growth factors in gastric cancer. J Surg Res. 2015;194:420-9.
  30. Nassiri F, Cusimano MD, Scheithauer BW, Rotondo F, Fazio A, Yousef GM, et al. Endoglin (CD105): a review of its role in angiogenesis and tumor diagnosis, progression and therapy. Anticancer Res. 2011;31:2283-90.
  31. Himani B, Meera S, Abhimanyu S, Usha R. Ki-67 Immunostaining and its Correlation with Microvessel Density in Patients with Mutiple Myeloma. Asian Pac J Cancer Prev. 2016;17:2559-64. 
  32. Kerbel RS. Tumor angiogenesis: past, present and the near future. Carcinogenesis. 2000;21:505-15.
  33. Chopra A. 89Zr-Labeled anti-CD105 (endoglin) chimeric monoclonal antibody TRC105 linked to IRDye 800CW. Molecular Imaging and Contrast Agent Database (MICAD). Bethesda (MD)2004.
  34. Zhang Y, Yang Y, Hong H, Cai W. Multimodality molecular imaging of CD105 (Endoglin) expression. Int J Clin Exp Med. 2011;4:32-42.
  35. Aristorena M, Blanco FJ, de Las Casas-Engel M, OjedaFernandez L, Gallardo-Vara E, Corbi A, et al. Expression of endoglin isoforms in the myeloid lineage and their role during aging and macrophage polarization. J Cell Sci. 2014;127:2723-35.
  36. Bernabeu C, Lopez-Novoa JM, Quintanilla M. The emerging role of TGF-beta superfamily coreceptors in cancer. Biochim Biophys Acta. 2009;1792:954-73.
  37. Miyahara M, Tanuma J, Sugihara K, Semba I. Tumor lymphangiogenesis correlates with lymph node metastasis and clinicopathologic parameters in oral squamous cell carcinoma. Cancer. 2007;110:1287-94.
Alexandria Dental Journal
Volume 44, Issue 1
April 2019
Pages 117-122

Files

Share

How to cite

Statistics