THE EFFECT OF PLATELET RICH FIBRIN ON ANGIOGENESIS DURING PERIODONTAL REGENERATION

Samuel, Moody A.; Abou Khadr, Maha A.; Koura, Azza S.; Madi, Marwa I.

doi:10.21608/adjalexu.2017.57924

THE EFFECT OF PLATELET RICH FIBRIN ON ANGIOGENESIS DURING PERIODONTAL REGENERATION

Document Type : Original Article

Authors

¹ - Bachelor of Dental medicine and surgery 2007, Faculty of dentistry, Alexandria University, Egypt

² Professor of Periodontology, Alexandria University, Egypt.

³ Assistant Professor of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt

⁴ Lecturer of Periodontology, Faculty of Dentistry, Alexandria University, Egypt.

10.21608/adjalexu.2017.57924

Abstract

INTRODUCTION: Platelet rich fibrin (PRF) is an autologous healing biomaterial composed of a fibrin clot matrix entrapping leukocytes, cytokines, living progenitor cells, and platelets capable of releasing various growth factors harvested from a simple blood sample. OBJECTIVES: The aim of this experimental study was to evaluate the effect of PRF on angiogenesis during the periodontal regeneration of surgically created grade ΙΙ furcation defects in dogs. MATERIALS AND METHODS: A split mouth design was carried out using the third and fourth mandibular premolars of 2 healthy mongrel adult dogs. A total of eight grade II furcation defects were surgically created. The defects in the study side were augmented with PRF mixed with a β-TCP and covered by a PRF membrane and a collagen membrane. In the control side the defects were augmented with β-TCP and covered with a collagen membrane. The dogs were sacrificed after 1 and 2 months. Samples were dissected and prepared for histological evaluation. RESULTS: Histological results showed that PRF addition led to higher vascularization of the study samples at all age groups; this was evident at one month in the form of numerous blood vessels entrapping RBCs and at 2 months by the presence of red bone marrow and blood vessels entrapping RBCs within. CONCLUSIONS: The addition of PRF to β -TCP was found to be effective in enhancing angiogenesis during the periodontal regeneration of grade II furcation defects

Keywords

Main Subjects

References

1. Uchida S, Sakai A, Kudo H, Otomo H, Watanuki M, Tanaka M et al. Vascular endothelial growth factor is expressed along with its receptors during the healing process of bone and bone marrow after drill-hole injury in rats. Bone.2003;32: 491-501.

2. Wang CJ, Chen HS, Chen CE, Yang KD. Treatment of nonunions of long bone fractures with shock waves. Clin Orthop Relat Res. 2001; 95:101-9.

3. Wang CJ, Huang KE, Sun YC, Yang YJ, Ko JY, Weng LH et al. VEGF modulates angiogenesis and osteogenesis in shockwave-promoted fracture healing in rabbits. J Surg Res. 2011;171: 114- 9.

4. Davi G, Patrono C. Platelet activation and atherothrombosis. N Engl J Med. 2007; 357:2482–94.

5. Mehta D, Deshpande N, Dave D, Modi B, Bharwani A. Platelet rich fibrin: New treatment modality in Grade II furcation defects. J Oral Dis Market. 2016; 1:1-5.

6. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J et al. Platelet-rich fibrin (PRF): A secondgeneration platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 101:37-44.

7. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J et al. Platelet-rich fibrin (PRF): a secondgeneration platelet concentrate. Part II: platelet related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 101:45–50.

8. Toffler M, Toscano N, Holtzclaw D, Del Corso M, Ehrenfest DD. Introducing Choukroun’s platelet rich fibrin (PRF) to the reconstructive surgery, The Journal of Implant and Advanced Clinical Dentistry. 2009;6: 21–32.

9. Bowers GM, Schallhorn RG, McClain PK, Morrison GM, Morgan R, Reynolds MA. Factors influencing the outcome of regenerative therapy in mandibular class II furcations: Part I. J Periodontol 2003; 74:1255-1268.

10. Bölükbaşı N, Yeniyol S, Tekkesin MS, Altunatmaz K. The Use of Platelet-Rich Fibrin in Combination with Biphasic Calcium Phosphate in the Treatment of Bone Defects: A Histologic and Histomorphometric Study. Current Therapeutic Research, Clinical and Experimental. 2013; 75:15-21.

11. Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108: 56-61.

12. Yilmaz D, Dogan N, Ozkan A, Sencimen M, Oral BE, Mutlu I. Effect of platelet rich fibrin and beta tricalcium phosphate on bone healing.A histological study in pigs. Acta Cirúrgica Brasileira. 2014; 29:59-68.

13. Kökdere N, Baykul T, Findik Y. The use of platelet-rich fibrin (PRF) and PRF-mixed particulated autogenous bone graft in the treatment of bone defects: An experimental and histomorphometrical study. Dent Res J (Isfahan). 2015;12: 418–24.

14. Prakash S, Thakur A. Platelet concentrates: Past, present and future. J Maxillofac Oral Surg. 2011; 10:45–9.

15. Sunitha RV, Munirathnam NE. Platelet-rich fibrin: Evolution of a second-generation platelet concentrate Indian. J Dent Res. 2008;19: 42-6.

16. Steinbrech DS, Mehrara BJ, Saadeh PB, Greenwald JA, Spector JA, Gittes GK et al. VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am J Physiol Cell Physiol. 2000; 278: 853-60.

17. Yoon JS, Lee SH, Yoon HJ. The influence of platelet-rich fibrin on angiogenesis in guided bone regeneration using xenogenic bone substitutes: A study of rabbit cranial defects, Journal of Cranio-Maxillo-Facial Surgery. 2014; 34:1-7.

18. Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL et al: Platelet rich fibrin (PRF): a secondgeneration platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101: 299-303.