Sherif, Y., El Masry, N., Karam, S., Nasra, M. (2016). EVALUATION OF LOCAL ADMINISTRATION OF SIMVASTATIN ON HEIGHT AND WIDTH OF THE HEALING EXTRACTION SOCKET IN RAT MANDIBLE. Alexandria Dental Journal, 41(3), 283-286. doi: 10.21608/adjalexu.2016.58040
Youmna M. Sherif; Nawal El Masry; Sahar S. Karam; Maha A. Nasra. "EVALUATION OF LOCAL ADMINISTRATION OF SIMVASTATIN ON HEIGHT AND WIDTH OF THE HEALING EXTRACTION SOCKET IN RAT MANDIBLE". Alexandria Dental Journal, 41, 3, 2016, 283-286. doi: 10.21608/adjalexu.2016.58040
Sherif, Y., El Masry, N., Karam, S., Nasra, M. (2016). 'EVALUATION OF LOCAL ADMINISTRATION OF SIMVASTATIN ON HEIGHT AND WIDTH OF THE HEALING EXTRACTION SOCKET IN RAT MANDIBLE', Alexandria Dental Journal, 41(3), pp. 283-286. doi: 10.21608/adjalexu.2016.58040
Sherif, Y., El Masry, N., Karam, S., Nasra, M. EVALUATION OF LOCAL ADMINISTRATION OF SIMVASTATIN ON HEIGHT AND WIDTH OF THE HEALING EXTRACTION SOCKET IN RAT MANDIBLE. Alexandria Dental Journal, 2016; 41(3): 283-286. doi: 10.21608/adjalexu.2016.58040
EVALUATION OF LOCAL ADMINISTRATION OF SIMVASTATIN ON HEIGHT AND WIDTH OF THE HEALING EXTRACTION SOCKET IN RAT MANDIBLE
2PhD, Professor of oral biology - Faculty of Dentistry - Alexandria University
3PhD, Professor of oral biology - Faculty of Dentistry - Alexandria University.
4PhD, Lecturer of Pharmaceutics-Faculty of Pharmacy-Alexandria University.
Abstract
INTRODUCTION: studies concerning healing of extraction sockets revealed that sockets were filled with new bone by as much as two thirds in 40 days and completely filled with new bone in 10 weeks. There have been many studies demonstrating the bone-promoting effect of simvastatin local application in animal models. Simvastatin was shown to increase bone volume, bone formation rate, and bone compressive strength. The use of statins for bone regeneration is a promising and growing area of research. OBJECTIVES: The aim of this study was to compare between the amounts of resorption that occurs in the healing extraction socket left to heal spontaneously to the amount of resorption that occurs in the healing extraction socket filled with simvastatin gel. MATERIALS AND METHODS: Right and left first molars were extracted from 20 rats’ mandibles’. Right side extraction sockets (Experimental) were filled with 2.5% simvastatin gel, while the left side sockets (Controls) were allowed to heal spontaneously. The rats were humanely sacrificed at 1st,2nd,3rd and 4th week postoperatively, and the specimens’ height and width were measured using a bone caliper. RESULTS: The mid-buccal height as well as the buccolingual width of the alveolar bone on the simvastatin-treated side was relatively higher and thicker than the control side indicating that less resorption had occurred on that side. CONCLUSIONS: The present study provided evidence that a single topical application of 2.5% simvastatin gel improve the quality of the new bone of the healing extraction socket and decreases bone resorption.
1. DrakeMT,ClarkeBL,KhoslaS.Bisphosphonates: mechanism of action and role in clinical practice. Mayo Clin Proc 2008; 83: 1032-45. 2. EndoA.Ahistoricalperspectiveonthediscoveryofstatins. Proc Jpn Acad Ser B 2010; 86: 484-93. 3. MundyG,GarrettR,HarrisS,ChanJ,ChenD,RossiniG, et al. Stimulation of bone formation in vitro and in rodents by statins. Science 1999; 286: 1946-9. 4. Marie PJ, Debiais F, Hay E. Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol Histopathol 2002; 17: 877-85.
5. Zhang Y, Bradley AD, Wang D, Reinhardt RA. Statins, bone metabolism and treatment of bone catabolic diseases. Pharmacol Res 2014; 88: 53-61. 6. Marks SC, Hermey DC. The structure and development of bone. In: Bilezekian JP, Raisz LG, Rodan GA, editors. Principles of bone biology. San Diego: Academic Press; 1996: 3–24 7. Huebsch RF, Hansen LS. A histopathologic study of extraction wounds in dogs. Oral Surg Oral Med Oral Pathol 1969; 28:187–96 8. Guglielmotti MB, Cabrini RL. Alveolar wound healing and ridge remodeling after tooth extraction in the rat. a histologic, radiographic and histometric study. J Oral Maxillofac Surg 1985; 43: 359-64. 9. SmithN.Acomparativehistologicalandradiographicstudy of extraction socket healing in rat. Australian Dental J 1974; 250-4. 10.Woo JT, Kasai S, Stern PH, Nagai K. Compaction suppresses bone resorption by inhibiting the fusion of perfusion osteoclasts and disrupting the actin ring in osteoclasts. J Bone Miner Res 2000; 15: 650-62. 11. Fiedler J, Leucht F, Waltenberger J, Dehio C, Brenner RE. et al. VEGF-A and PlGF-1 stimulate chemotactic migration of human mesenchymal progenitor cells. Biochem Biophys Res Commun 2005; 334: 561-8. 12. Costa-Pinto AR, Reis RL, Neves NM. Scaffolds based bone tissue engineering: the role of chitosan. Tissue Eng Part B Rev. 2011; 17:331–347. 13.Parfitt AM. The cellular basis of bone remodeling: the quantum concept reexamined in light of recent advances in the cell biology of bone. Calcif Tissue Int 1984; 36: S37-45. 14.Grasser WA, Baumann AP, Petras SF, Harwood HJ, Devalajara R, Renkiewicz R, et al. Regulation of osteoclast differentiation by statins. J Musculoskelet Neuronal Interact 2003; 3: 53-62. 15. Perchyonok VT, Reher V, Zhang S, Basson N, Grobler S. Evaluation of Nystatin Containing Chitosan Hydrogels as Potential Dual Action Bio-Active Restorative Materials: in Vitro Approach. J Funct Biomater 2014; 5: 259-72. 16. Ismail FA. Design and in vitro evaluation of polymeric formulae of simvastatin for local bone induction. Drug Dev Ind Pharm 2006; 32: 1199-206. 17. Kiliaridis, S.; Thilander, B.; Kjellberg, H.; Topouzelis, N. & Zafiriadis. Effect of low masticatory function on condylar growth: a morphometric study in the rat. Am. J. Orthod. Dentofacial Orthop., 1999: 116:121-5. 18.Yazawa AH, Zimmermann B, Asami Y, Bernimoulin JP. Simvastatin promotes cell metabolism, proliferation, and osteoblastic differentiation in human periodontal ligament cells. J Periodontol 2005; 76: 295-302.