Shama, M., Aboukhadr, M., Madi, M., Abdelhady, S. (2016). COMPARISON BETWEEN LEVEL OF INTEREUKIN 10 IN THE GINGIVAL CREVICULAR FLUID AND PERI-IMPLANT SULCULAR FLUID AROUND HEALTHY DENTAL IMPLANTS (SPLIT MOUTH STUDY). Alexandria Dental Journal, 41(1), 26-30. doi: 10.21608/adjalexu.2016.59168
MM Shama; M Aboukhadr; M Madi; S Abdelhady. "COMPARISON BETWEEN LEVEL OF INTEREUKIN 10 IN THE GINGIVAL CREVICULAR FLUID AND PERI-IMPLANT SULCULAR FLUID AROUND HEALTHY DENTAL IMPLANTS (SPLIT MOUTH STUDY)". Alexandria Dental Journal, 41, 1, 2016, 26-30. doi: 10.21608/adjalexu.2016.59168
Shama, M., Aboukhadr, M., Madi, M., Abdelhady, S. (2016). 'COMPARISON BETWEEN LEVEL OF INTEREUKIN 10 IN THE GINGIVAL CREVICULAR FLUID AND PERI-IMPLANT SULCULAR FLUID AROUND HEALTHY DENTAL IMPLANTS (SPLIT MOUTH STUDY)', Alexandria Dental Journal, 41(1), pp. 26-30. doi: 10.21608/adjalexu.2016.59168
Shama, M., Aboukhadr, M., Madi, M., Abdelhady, S. COMPARISON BETWEEN LEVEL OF INTEREUKIN 10 IN THE GINGIVAL CREVICULAR FLUID AND PERI-IMPLANT SULCULAR FLUID AROUND HEALTHY DENTAL IMPLANTS (SPLIT MOUTH STUDY). Alexandria Dental Journal, 2016; 41(1): 26-30. doi: 10.21608/adjalexu.2016.59168
COMPARISON BETWEEN LEVEL OF INTEREUKIN 10 IN THE GINGIVAL CREVICULAR FLUID AND PERI-IMPLANT SULCULAR FLUID AROUND HEALTHY DENTAL IMPLANTS (SPLIT MOUTH STUDY)
INTRODUCTION: Several methods are used in monitoring peri-implant clinical status most of these are periodontal in origin, unfortunately these methods only determine established disease rather than assessing early inflammatory response. Recently, Peri-implant sulcular fluid analysis become a major important index in monitoring peri-implant clinical status. OBJECTIVE: The aim of this study was to compare and evaluate levels of interleukin 10 in the peri-implant sulcular fluid and the gingival crevicular fluid. MATERIALS AND METHODS: Sixteen patients with functionally osseointigrated dental implants were conducted in this split mouth study. Crevicular fluid was collected by absorbent paper points, Interleukin 10 levels were evaluated by using ELISA method for interleukin 10 level from two sites in each subject,study sites: around dental implant and control sites: around antagonist tooth in the same arch. Specimens were evaluated by ELISA method for interleukin level. RESULTS: The levels of IL-10 for neobiotech dental implant showed a mean of 4.45±0.55 in the PISF and 2.85±0.34 GCF and for astratech dental implant IL10 showed a mean of 3.35±0.44 in PISF and 2.75± 0.41 in GCF. Levels of IL10 for the implant systems showed statistically significant higher levels in the PISF than GCF (P<0.05). CONCLUSION: IL-10 can be used as site specific marker in monitoring early inflammatory changes in both gingival crevicular fluid &peri-implant sulcular fluid.
1. Beatner R, Mihacl J, Schwenzer B. Biological manofunctionalization of titanium based biomaterial surfaces on Osseointigration: a flexible tool box. J R Soc. Interface 2010;7:93-105.
2. Branemark PI, ZarbGA,Albertsson T. Tissue integrated prosthesis. Osseointigration in clinical dentistry. Chicago: Quintessence publishing Co. Inc; 1985.
3. Heasmann P. Periimplant disease. Dent update. 2010;37:511-2.
4. Medzhitov R. Recognition of microorganisms and activation of immune response. Nature. 2007;449:819-26.
5. Berglundh T, Lindhe J, Erissson I, Mariello CP, Thamsen P.The soft tissue Barrier at implants and teeth.Clin Oral Implants Res. 1991;2:81-90.
6. Comute AA, Weber HP,Shortkroff S, Cui FZ, Spector M. Connective tissue orientation around dental implants in Cannine model. Clinical Oral Implant Res. 2001;12:433-40.
7. Hannig M. Transmission electron microscope study of in vivo pellicle formation on dental restorative materials. Eur J Oral Sci. 1997;105:422-33.
8. Lindhe J, Berglundh T, Ericsoon I. Experimental breakdown of peri-implant and periodontal tissues. A study on beagle dog.Clin oral Implants Res. 1992;3:9-16.
9. Albrektsson T, Zarb G, Worthington P, Eriksson AR. The longterm efficacy of currently used dental implants: a review and proposed critera for success. Int. J Oral Maxillofac Implants. 1986;1:11-25.
10. Löe H, Sillncss J, Periodontal disease in Pregnancy. 1. Pervalence and Severity.ActaOdontol Scand. 1963;21:533-51.
11. Amato R, Caton J, Polson A, Espeland M. Interporxinot gingival inflammation related to the conversion of a bleeding to a non bleeding state. J Periodontol. 1986;57:63-8.
12. Greenstein G. Contemporary interpretation of probing depth assessments: Diagnostic and therapeutic indications. J Periodontol. 1996;68:1194-205.
13. American Academy of Periodontology.Glossary of periodontal terms. Chicago: American Academy of Periodontology; 2001.
14. Apse P, Ellen RP, Overall CM, Zarb GA. Microbiota and crevicular fluid collagenase activity in the Osseointigrated dental implant sulcus: a comparison of sites in edentulous and partially edentulous patients. J Periodontal Res. 1989;24:96- 105.
15. Lang NP, Berglundh T. Woring Group 4 of seventh Euroup Workshop on Periodontology. Peri-implant diseases: Where are we now? Consensus of the seventh European workshop on periodontology.J ClinPeriodontol. 2011;38(suppl 11):178-81.
16. Schierano G, Pejrone G, Brusco P, Preti G. TNFalpha TGF-beta 2 and IL-1 beta levels in gingival and peri-implant crevicular fluid before and after de novo plaque accumulation. J ClinPeriodontal. 2008;35:532-8.
17. Tözüm TF, Turkyilmaz I, YamaliKN, Tümer C, Kilinc A, Kilinç K, et al. The effect of delayed versus early loading on nitric Oxide metabolism around dental implants: an 18-months comparative followup study. Int J Oral Maxilloface Implants. 2007;22:53-62.
18. Bondin S, Flemmig TF, Verraoli S. Role of fibroblast population in peri-implantitis, Int J Oral Maxillofac Implants. 2009;24:197-204.
19. Sasaki H, Hou L, Belani A, Wang CY, Uchiyama T, Müller R, et al. IL-10, but not IL-4, suppresses infection-stimulated bone resorption in vivo. J Immunol. 2000;165: 3626-30.
20. Defrance T, Vanbervleit B, Briere F, Durand I, Rousset F, Banchereau J. Interleukin 10 and transforming growth factor beta cooperate to induce anti-CD 40-activated naïve human b cells to secrete immunoglobulin A. J Exp Med. 1992;175:671-82.
21. Hijeer AH, Lazarus M, Turner D. Mageed RA, VencovsKyJ, Sinnott P, etal. IL 10 gene promoter polymorphisms in rheumatoid arthritis, Scand J Rheumatol. 1998;27:142-5.
22. Jinquiqn T, Larsen CG, Gesser B, Matsushima K, Thestrup-Pedersen K. Human IL-10 is a chemo attractant for CD8+T Lymphocytes and an inhibitor of IL-8 induced CD4+T lymphocyte migration. J Immunol. 1992;151:4545-51.
23. Owens JM, Gullagher AC, Chambers TJ. IL-10 modulates formation of osteclasts in murine hemopoietic cultures. J Immunol. 1996;157:936- 40.
24. MosmannT. Measurement of mouse and human interleukin-10. CurrProtocImmunol. 2001;Chapter 6:Unit 6.14.
25. Kirkpatrick LA, Feeney BC. A simple guide to IBM SPSS statistics for version 20.0. Student ed. Belmont, Calif.: Wadsworth, Cengage Learning; 2013.
26. Pietruski JK, Pietruski S, Stokowska W, Pattarelli GM. Serum levels of IL 1, IL 6 and IL8 in patients treated with dental implants. RoczAkad Med Bialynst. 2001;46:28-37.
27. Paul N.E, Shazikc, Harwardt M, Klee D, Salber J. Topographical control of human macrophages by a regularly micro-structured poly ringlidene fluoride surface. Biomaterials. 2008;29:4056-64.
28. Broggini N, McManus M, Hermann S. Peri-implant inflammation defined by the implant abutment interface. J Dental Res. 2006;85:473-8.
29. Liskmann S, Vihalemm T, Salum O, Zilmer K, Fischer K, Zilmer M. Correlations between clinical parameters and interleukin-6 and interleukin-10 levels in saliva from totally edentulous patients with peri-implant disease. Int J Oral Maxillofac Implants. 2006;21:543-50.
30. Sasaki H, Okamatsu Y, Kuwai T, Kent R, Taubman M, Stashenko P. The IL 10 knockout mouse is highly susceptible to Prophymonousgingivalis induced alveolar bone loss. J Periodontal Res. 2004;39:432-41.
31. Schierano G, Bellone G, Cassarino E, Pagano M, Preti G, Emanuelli G. Transforming growth factor beta and interleukin 10 in Oral implant sites. J Dent Res. 2003;82:428-32.
32. Guliz N, Abdullah C, Sevim G, Ezel B. Effect of inflammation on cytokine levels and bone remodeling markers in the peri implant fluid: a preliminary report. Int J oral Maxillofac Implants. 2012;59:313-6.
33. Casado PL, De Almeida A, Barboza EP, Duarte ME. Interleukin 1 beta and Interleukin 10 expressions in the peri implant crevicular fluid from patients with untreated peri implant disease. J Implant Dent. 2013;22:143-50.
34. Nogueira-Filho G, Pesun I, Isaak-Ploegman C, Wijegunasinghe M, Wierzbicki T, McCulloch CA. Longitudinal comparison of cytokines in Peri implant fluid and gingival crevicular fluid in healthy mouths. J Periodontol. 2014;85:1582-8.