EVALUATION OF NIGELLA SATIVA ON SOCKET HEALING IN RABBITS

Document Type : Original Article

Authors

1 Demonstrator of Oral Biology,, Faculty of Dentistry, Alexandria University, Egypt.

2 Professor of Oral Biology, Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.

Abstract

INTRODUCTION: Tooth extraction socket healing is a complex process, which involves tissue repair and regeneration. Concurrent with bone growth into the socket, there is also well-documented resorption of the alveolar ridges. Therefore, the socket preservation concept was introduced. One of the top ranked evidence-based herbal medicines, which has been described as the “miracle herb of the century” is Nigella sativa (NS). N. sativa has been extensively studied for its biological activities and therapeutic potential and shown to possess wide spectrum of activities. OBJECTIVES: To evaluate the biological effect of Nigella sativa on socket healing in rabbits. MATERIALS AND METHODS: Twelve healthy rabbits were used in this study, lower first premolars (right and left) were extracted. All right sockets were left for normal healing as a control group, while the left sockets are considered as the study group and they were treated with Nigella Sativa. All sockets were sutured. Rabbits were sacrificed after two weeks. Their mandibles were dissected out to be processed for histological examination under the light microscope. RESULTS: In the control group, the histological examination of the socket revealed randomly arranged thin bony spicules lined by flattened layer of osteoblast cells on its surface, enclosing fibrous bone marrow with limited vascularity. Osteocytes were of moderate size and density. In the Nigella sativa treated group sockets exhibited more active bone formation, thick trabeculae with highly vascular bone marrow and large numerous osteocytes. Certain areas show replacement of woven bone with mature bone where bone remodeling takes place. CONCLUSIONS: Use of Nigella sativa after extraction in the healing sockets could enhance bone formation and accelerate the process of socket healing

Keywords

Main Subjects

References

  1. Hupp JR, Ellis E, Tucker MR. Contemporary oral and maxillofacial surgery. 5th ed. St. Louis, Mo.: Mosby Elsevier; 2008.
  2. Lalani Z, Wong M, Brey EM, Mikos AG, Duke P. Spatial and temporal localization of transforming growth factor-b1, bone morphogenetic protein-2, and platelet-derived growth factor-A in healing tooth extraction sockets in a rabbit model. J Oral Maxillofac Surg.2003;61:1061-72.
  3. Cardaropoli G, Araújo M, Lindhe J. Dynamics of bone tissue formation in tooth extraction sites. An experimental study in dogs. J Clin Periodontol.2003;30:809-18.
  4. Guo S, DiPietro LA. Factors affecting wound healing. J Dent Res.2010;89:219-29.
  5. Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res.2009;37:1528-42.
  6. Chen ST, Wilson Jr TG, Hammerle C. Immediate or early placement of implants following tooth extraction: review of biologic basics, clinical procedures, and outcomes. Int J Oral Maxillofac Implants.2004;19:12-25.
  7. Iizuka T, Miller SC, Marks SC. Alveolar bone remodeling after tooth extraction in normal and osteoporotic (ia) rats. J Oral Pathol Med.1992;21:150-5.
  8. Van der Weijden F, Dell’Acqua F, Slot DE. Alveolar bone dimensional changes of postextraction sockets in humans: a systematic review. J Clin Periodontol.2009;36:1048-58.
  9. Peck MT1, Marnewick J, Stephen L. Alveolar Ridge Preservation Using Leukocyte and Platelet-Rich Fibrin: A Report of a Case. Case Rep Dent.2011:1-5.
  10. Chen ST. Clinical and Esthetic Outcomes of Implants Placed in Postextraction Sites. Int J Oral Maxillofac Implants.2009;24:186-2.
  11. Jamjoom A, Cohen RE. Grafts for Ridge Preservation. J Funct Biomater.2015;6:833-48.
  12. Sogut B, Celik I, Tuluce Y. The effect of diet supplemented with the Black cumin upon Immune potential and antioxidant marker enzymes and lipid peroxidation in broiler chicks. J Anim Vet Adv.2008;7:1196-9.
  13. Ahmad I, Tripathi J, Sharma M, Karchulli MS, Umer L. Nigella sativa - a medicinal herb with immense therapeutic potential (a systematic review). Int J Biol Pharma Res. 2014;5:755-62.
  14. Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed. 2013;3:337-52
  15. El-Tahir KH, Bakeet D. The Black seed Nigella Sativa: Aplea for urgent clinical evaluation of its volatile oil. JTU Med Sci.2006;1:1-19.
  16. Orban BJ, Bhaskar SN. Oral histology and embryology.11th ed. Saint Louis: Mosby;1991.
  17. Goreja WG. Black seed: nature's miracle remedy. New York, NY: Amazing Herbs Press; 2003.
  18. Kuboki Y, Hashimoto F, Ishibashi K. Time dependent changes of collagen cross links in the socket after tooth extraction in rabbits. J Dent Res.1988;67:944-8.
  19. Centrella M, McCarthy TL, Canalis E. Effects of transforming growth factors on bone cells. Con Tiss Res.1989;20:267-75.
  20. Joyce ME, Jingushi S, Bolander ME. Transforming growth factor-8 in the regulation of fracture repair. Orthop Clin North Am.1992;21:199-209
  21. Nakase T, Nomura S, Yoshikawa H, Hashimoto J, Hirota S, Kitamura Y, et al. Transient and localized expression of bone morphogenetic protein 4 messenger RNA during fracture healing. J Bone Miner Res.1994;9:651-9.
  22. Sandberg MM. Matrix in cartilage and bone development: current views on the function and regulation of major organic components. Ann Med.1991;23:207-17.
  23. Eatelaf A. The percutaneous effect of black seed (Nigella Sativa) oil as external topical treatment on bone healing in rabbits. QJVMS.2014;13:146-54.
  24. Arslan AH, Tomruk CÖ, Meydanlı EG, Özdemir İ, Duygu Çapar G, Kütan E, et al. Histopathological evaluation of the effect of systemic thymoquinone administration on healing of bone defects in rat tibia. Biotechnol Biotec Eq.2017;31:175-81.
  25. Akhtar M, Maikiyo AM, Khanam R, Mujeeb M, Aqil M, Najmi AK. Ameliorating effects of two extracts of Nigella sativa in middle cerebral artery occluded rat. J Pharm Bioallied Sci.2012;4:70-5.
  26. Gerber HP, Vu TH, Ryan AM, Kowalski J, Werb Z, Ferrara N.VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation. Nat Med.1999;5:623-8.
  27. Glowacki J. Angiogenesis in fracture repair. Clin Orthop Relat Res.1998;355:S82-9.
  28. Al Asoom LI. Coronary angiogenic effect of long-term administration of Nigella sativa. BMC Complement Altern Med. 2017;17:308.
  29. Zheng W, Seftor EA, Meininger CJ, Hendrix MJ, Tomanek RJ. Mechanisms of coronary angiogenesis in response to stretch: role of VEGF and TGF-beta. Am J Physiol Heart Circ Physiol.2001;280:H909-17.
  30. Kolmakova A, Rajesh M, Zang D, Pili R, Chatterjee S. VEGF recruits lactosylceramide to induce endothelial cell adhesion molecule expression and angiogenesis in vitro and in vivo. Glycoconj J.2009;26:547-58.
  31. Randi AM, Laffan MA, Starke RD. Von Willebrand factor, angiodysplasia and angiogenesis. Mediterr J Hematol Infect Dis.2013;5:e2013060.
  32. Starke RD, Ferraro F, Paschalaki KE, Dryden NH, McKinnon TA, Sutton RE, et al. Endothelial von Willebrand factor regulates angiogenesis. Blood.2011;117:1071-80.
Alexandria Dental Journal
Volume 44, Issue 3
December 2019
Pages 60-64

Files

Share

How to cite

Statistics