EVALUATION OF ANTIMICROBIAL EFFECT OF TWO PROPOLIS FORMULAE ON STREPTOCOCCUS MUTANS AND CANDIDA ALBICANS: A RANDOMIZED CLINICAL TRIAL

Document Type : Original Article

Authors

1 Demonstrator of Pediatric Dentistry, Pediatric Dentistry Department, Faculty of Dentistry, Benghazi University, Libya.

2 Professor of Pediatric Dentistry, Pediatric Dentistry and Dental Public Health Department, Faculty of Dentistry, Alexandria University, Egypt.

3 Associate Professor of Pediatric Dentistry, Pediatric Dentistry and Dental Public Health Department, Faculty of Dentistry, Alexandria University, Egypt.

4 Associate Professor of Microbiology and Immunology, Microbiology and Immunology Department, Faculty of Pharmacy, Alexandria University, Egypt

Abstract

INTRODUCTION: Streptococcus mutans is considered to be the most cariogenic microorganism. However, candida albicans has also been isolated from dental plaque of high caries risk children. Therefore, uses of natural antimicrobial agents such as propolis have been established as an adjunctive approach for oral health care.
OBJECTIVES: the aim of this study was to evaluate the antibacterial and antifungal effects of propolis chewing gum compared to propolis mouthwash.
MATERIALS AND METHODS: Sixty high caries risk children aged 6 to 8 were randomly assigned into two groups. Group I received propolis chewing gum and Group II received propolis mouthwash. A baseline and final plaque samples were obtained from each participant before and after intervention respectively within 14 days interval. All participants were asked to refrain from eating or brushing on the sampling days. Samples were sent immediately to the laboratory for microbial assessment.
RESULTS: data revealed that both study groups showed significant differences (P≤0.001) in streptococcus mutans and candida albicans counts before and after intervention. But there was no significant difference among the comparative groups.
CONCLUSION: It was demonstrated that propolis chewing gum and propolis mouthwash can be used as antimicrobial agents.

Keywords

Main Subjects

References

  1. Seneviratne CJ, Zhang CF, Samaranayake LP. Dental Plaque Biofilm in oral Health and Disease. Chinese J Dent Res. 2011;14:87–94.
  2. Thenisch NL, Bachmann LM, Imfeld T, Leisebach Minder T, Steurer J. Are Mutans Streptococci Detected in Preschool Children a Reliable Predictive Factor for Dental Caries Risk? A Systematic Review. Caries Res. 2006;40:366–74.
  3. Nikawa H, Yamashiro H, Makihira S, Nishimura M, Egusa H, Furukawa M, et al. In vitro cariogenic potential of Candida albicans. Mycoses. 2003;46:471–8.
  4. De Carvalho FG, Silva DS, Hebling J, Spolidorio LC, Spolidorio DMP. Presence of mutans streptococci and Candida spp. in dental plaque/dentine of carious teeth and early childhood caries. Arch Oral Biol. 2006;51:1024–8.
  5. Gurenlian JR. The Role of dental paque biofilm in oral health. J Dent Hyg. 2007;81:1–11.
  6. Weijden V Der, Kpk H. A systematic review of the effectiveness of self-performed mechanical plaque removal in adults with gingivitis using a manual toothbrush. J Clin Periodontol. 2005;32:214–28.
  7. Coelho Leal S, Barreto Bezerra AC, Ayrton de Toledo O. Effectiveness of teaching methods for toothbrushing in preschool children. Braz Dent J. 2002;13:133–6.
  8. Gunsolley JC. A meta-analysis of six-month studies of antiplaque and antigingivitis agents. JADA. 2006;137:1649–57.
  9. Van der Weijden FA, Van der Sluijs E, Ciancio SG, Slot DE. Can Chemical Mouthwash Agents Achieve Plaque/Gingivitis Control? Dent Clin North Am. 2015;59:799–829.
  10. James P, Worthington H V, Parnell C, Harding M, Lamont T, Cheung A, et al. Chlorhexidine mouthrinse as an adjunctive treatment for gingival health. Cochrane Database Syst Rev. 2017 Mar 31;3.
  11. Zanatta FB, Antoniazzi RP, Rösing CK. Staining and calculus formation after 0.12% chlorhexidine rinses in plaque-free and plaque covered surfaces: a randomized trial. J Appl Oral Sci. 2010;4:515–21.
  12. Pemberton MN, Gibson J. Chlorhexidine and hypersensitivity reactions in dentistry. Br Dent J. 2012;213:547–50.
  13. Malhotra S, Gupta V. Use of propolis in pediatric dentistry. J Dent Allied Sci. 2014;3:93–8.
  14. Kujumgieva A, Tsvetkovaa I, Serkedjievaa Y, Bankovab V, Christovb R, Popovb S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol. 1999;64:235–40.
  15. Betances-Salcedo E, Revilla I, Vivar-Quintana A, González-Martín M. Flavonoid and Antioxidant Capacity of Propolis Prediction Using Near Infrared Spectroscopy. Sensors. 2017;17:1647–59.
  16. Mossalayi MD, Rambert J, Renouf E, Micouleau M, Mérillon JM. Grape polyphenols and propolis mixture inhibits inflammatory mediator release from human leukocytes and reduces clinical scores in experimental arthritis. Phytomedicine. 2014;21:290–7.
  17. Miguel MG, Antunes MD. Is propolis safe as an alternative medicine? J Pharm Bioallied Sci. 2011;3:479– 95.
  18. de Camargo Smolarek P, Esmerino LA, Chibinski AC, Bortoluzzi MC, dos Santos EB, Kozlowski VA. In vitro antimicrobial evaluation of toothpastes with natural compounds. Eur J Dent. 2015;9:580–6.
  19. Siqueira AB, Rodriguez L, Santos RK, Marintho RR, Abreu S, Peixoto RF, et al. Antifungal activity of propolis against Candidaspecies isolated from cases of chronic periodontitis. Braz Oral Res. 2015;29:1–6.
  20. Pereira E, Silva J, Silva F, Luca M, Ferreira E, Lorenz T, et al. Clinical evidence of the efficacy of a mouthwash containing propolis for the control of plaque and gingivitis: A phase II study. Evidence-based Complement Altern Med. 2011;2011:1–7.
  21. Mickenautsch S, Leal SC, Yengopal V, Bezerra AC, Cruvinel V. Sugar-free chewing gum and dental caries: a systematic review. J Appl Oral Sci. 2007;15:83–8.
  22. Dodds MWJ. The oral health benefits of chewing gum. J Ir Dent Assoc. 2012;58:253–61.
  23. Ercan N, Erdemir EO, Ozkan SY, Hendek MK. The comparative effect of propolis in two different vehicles; mouthwash and chewing-gum on plaque accumulation and gingival inflammation. Eur J Dent. 2015;9:272–6.
  24. Rubido S, Fernández-Feijoo J, Limeres J, GarcíaCaballero L, Abeleira MT, Diz P. In vivo antiplaque effect of three edible toothpastes. Med Oral Patol Oral Cir Bucal. 2014;19:88–92.
  25. Faul F, Erdfelder E, Lang AG, Buchner A. G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods. 2007;39:175–91.
  26. American Academy of Pediatric Dentistry. Guideline on Caries-risk Assessment and Management for Infants, Children, and Adolescents. Clin Guidel Ref Man 2015- 2016. 2015;37:132–9.
  27. Frankl SN, Shire FR FH. Should the parent remain with the child in the dental operatory? J Dent Child. 1962;29:150–63.
  28. Imfeld T. Chewing gum - Facts and fiction: A review of gum-chewing and oral health. Crit Rev Oral Biol Med. 1999;10:405–19.
  29. Al-Hasani M, Hanno A, Dowidar K, Mostafa O, Soliman S. Effectiveness of Egyptian Propolis on Dental Plaque Formation in High Caries. Alexandria Dent J. 2016;41:194–8.
  30. Denbesten P, Hee M, Ko S. Fluoride levels in whole saliva of preschool children after brushing with 0.25 g (pea-sized) as compared 1.0 g (full-brush) of a fluoriiIe dentifrice. Pediatr Dent. 1996;182:77–80.
  31. Li J, Helmerhorst EJ, Leone CW, Troxler RF, Yaskell T, Haffajee AD, et al. Identification of early microbial colonizers in human dental biofilm. J Appl Microbiol. 2004;97:1311–8.
  32. Syed M, Chopra R, Shrivastava V, Sachdev V. Comparative evaluation of 0.2% Chlorhexidine Mouthwash, Xylitol Chewing Gum, and Combination of 0.2% Chlorhexidine Mouthwash and Xylitol Chewing Gum on Salivary Streptococcus mutans and Biofilm Levels in 8- to 12-Year-Old Children. Int J Clin Pediatr Dent. 2016;9:313–9.
  33. Yang Qiong X, Zhang Q, Lu Ying L, Yang R, Liu Y, Zou J. Genotypic distribution of Candida albicans in dental biofilm of Chinese children associated with severe early childhood caries. Arch Oral Biol. 2012;57:1048–53.
  34. Elnakady YA, Rushdi AI, Franke R, Abutaha N, Ebaid H, Baabbad M, et al. Characteristics, chemical compositions and biological activities of propolis from Al-Bahah, Saudi Arabia. Sci Rep. 2017;7:1–13.
  35. Sedgwick P. Intention to treat analysis versus per protocol analysis of trial data. BMJ. 2015;350:681.
  36. Drago L, De Vecchi E, Nicola L, Gismondo MR. In vitro antimicrobial activity of a novel propolis formulation (Actichelated propolis). J Appl Microbiol. 2007;103:1914–21.
  37. Hegde K, Bhat S, Rao A, Sain S. Effect of a propolis extract on Streptococcus mutans counts in vivo. Int J Clin Pediatr Dent. 2013;6:420–3.
  38. Ibrahim N, Zakaria AJ, Ismail Z, Mohd KS. Antibacterial and phenolic content of propolis produced by two Malaysian stingless bees, Heterotrigona itama and Geniotrigona thoracica. Int J Pharmacogn Phytochem Res. 2016;8:156–61.
  39. Duailibe SA de C, Gonçalves AG, Ahid FJM. Effect of a propolis extract on Streptococcus mutans counts in vivo. J Appl Oral Sci. 2007;15:420–3.
  40. Herrera CL, Alvear M, Barrientos L, Montenegro G, Salazar LA. The antifungal effect of six commercial extracts of Chilean propolis on Candida spp. Cienc e Investig Agrar. 2010;37:75–84. 
Alexandria Dental Journal
Volume 45, Issue 2
August 2020
Pages 99-103

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