EFFECT OF CALCITONIN INTRA MUSCULAR INJECTION ON BONE FORMATION AROUND TITANIUM DENTAL IMPLANTS IN DOGS (A HISTOLOGICAL STUDY)

Document Type : Original Article

Authors

1 Dentist, Faculty of Dentistry, Alexandria University, Egypt

2 Professor of Oral and Maxillofacial surgery, Faculty of Dentistry, Alexandria University, Egypt

3 Professor of Oral and Maxillofacial surgery, Faculty of Dentistry, Alexandria University, Egypt.

Abstract

INTRODUCTION: Calcitonin is a major hormone secreted by thyroid gland responsible for bone formation and decrease of blood calcium level. It is a mixture of amino acids plus a modifying agent. It has been proved that the synthetic salmon calcitonin enhanced bone growth in depth as well as in width that is why they used it as a treatment for bone diseases and fractures. OBJECTIVES: This study was done to evaluate histologically the effect of intra muscular (IM) calcitonin administration on bone healing around titanium dental implants. MATERIALS AND METHODS: Twelve adult’s Mongrel dogs received one implant in the maxilla in the edentulous space distal to the canine. Six dogs were randomly selected as the test group then they received a single daily dose of calcitonin 50 IU administered IM, for 28 successive days. And six dogs served as control group and no medications were injected. The animals were sacrificed 4, 8, and 12 weeks after surgery. RESULTS: Through microscopical examination of specimens showed an apparent trend toward increased new bone formation in the osteotomy site in the calcitonin-treated group compared with the controls in fourth and eighth week intervals. While in twelfth interval there was a non-significant difference could be noticed between control and study groups concerning new bone formation which gave firm attachment between host bone tissue and the used implant. CONCLUSIONS: This study demonstrated that using short, small dose of salmon calcitonin by daily intramuscular injection for 28 days enhanced bone mineral density when compared with the bone density without injection of this material.

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References

1. Branemark PI, Breine U, Adell R, Hanson BO, Lindströn J, Ohlsson Ä. Intra osseous anchorage of dental prostheses in experimental studies. Scand J Plast Reconstr Surg 1969; 3: 81-100. Quoted from: Januário AL, Sallum EA, de Toledo S, Sallum AW, Nociti JF Jr. Effect of calcitonin on bone formation around titanium implant. a histometric study in rabbits. Braz Dent J 2001; 12: 158-62. 
2. Guillermon E, Eric A Peter E, Michael B, Edwin A. Effect of alendronate on endosseous implant integration: An in vivo study in rabbits. J Oral Maxillofac Surg 2006; 64:2005- 9. 
3. Cornelini R, cangini F, Covani U, Barone A, Buser D. Immediate restoration of single tooth implants in mandibular molar sites: A 12-month preliminary report. Int J Oral Maxillofac Implants 2004; 19:855-60. 
4. Misch CE, Perel ML, Wang HL, Smmartino G, GaldinoMoreno P, Trisi P, et al. Implant success, survival, and failure: the international Congress of Oral Implantologists (ICOI) Pisa Consensus Conference. Implant Dent 2008; 17: 5-15. 
5. Sugerman PB, Barber M. Patient selection for endosseous dental implants: oral and systemic considerations. Int J Oral Maxillofac Implants 2002; 17: 191-201. 
6. Tarnow DP, Cho SC, Wallace SS. The effect of interimplant distance on the height of inter-implant bone crest. J Periodontal 2000; 71: 546-9. 
7. Fontana S, Oimedo D, Linares J, Gulielmotti M, Crosa M. Effect of platelet-rich plasms on peri-implant bone response; An experimental study. Impl Dent 2004; 13: 37- 8. 
8. Giavares G, Fini M, Gnudi S, Aldini N, Rocca M, Carpi A, et al. Comparison of calcitonin, alendronate and fluorophosphates effects on ovariectomized rat bone. Biomed Pharmacother 2001; 55: 397-403. 
9. Copp D, Cameron EC, Cheney BA, Davidson AG, Henze KG. Evidence for calcitonin a new hormone from the parathyroid gland that lowers blood calcium. Endocrinology 1962; 70: 638-49. Quoted from: Felsenfeld AJ, Levine BS. Calcitonin, the forgotten hormone: does it deserve to be forgotten? Clin Kidney J 2015; 8: 180-7. 
10. Gonzalez D, Ghiringhell G, Mautalen C. Acute antiosteoclastic effort of salmon calcitonin in osteoporotic women. Calcif Tissue Int 1986; 38:71-5. 
11. Lyritis GP, Taskalakos N, Magiasis B, Karachalios T, Yiatzides A, Tsekoura M. Analgesic effect of salmon calcitonin on osteoprotic vertebral fractures: a double-blind placebo-controlled clinical study. Calcif Tissue Int 1991; 49: 369-72. 
12. Glajchen N, Thomas S, Jowell P, Epstein S, Ismail F, Fallon M. The effect of high dose salmon calcitonin on bone mineral metabolism in rats. Calcif Tissue Int 1990; 46: 28-32. 
13.Reginster JY. Effect of calcitonin on bone mass and fracture rates. Am J Med 1991: 91: 195-225. 
14. Ysander M, Brånemark R, Olmarker K, Myers RR. Intermedullary osteointegration: development of rodent model and study of histology and neuropeptide changes around titanium implants. J Rehabil Res Dev 2001; 38: 183- 90. 
15. Mc Whinnie, D. In vivo effects of mammalian thyrocalcitonin on bone growth and alkaline phosphatase activity in the chick embryo.Comp Biochem physical 1975;50, 169-175. 
16. Guizhen J, Hiroko M, Junichi Y. Prevention of trabecular bone loss in the mandible of ovariectomized rats. J Oral Sci 2004; 46: 75-85. 
17. Dogan H, Ozcelik B, Gedikoglu G, Sensel S. The effect of calcitonin on osseous healing in guinea pig mandible. J Endod 2001; 27: 160-3. 
18.Januário AL, Sallum EA, de Toledo S, Sallum AW, Nociti JF Jr. Effect of calcitonin on bone formation around titanium implants (a histometric study in animals): Braz Dent J 2001; 12: 158-62. 
19. Pearce AI, Richards RG, Milz S, Schneider E, Pearce SG. Animal models for implant biomaterial research in bone: a review. Eur Cell Mater. 2007; 13:1-10. 
20. Sun Z, Herring SW, Tee BC, Gales J. Alveolar ridge reduction after tooth extraction in adolescents: An animal study. Arch Oral Biol. 2013; 58: 813-25. 
21. Mundt T, Mack F, Schwahn C, Biffar R. Private practice results of screw-type tapered implants: survival and evaluation of risk factors. Int J Oral Maxillofac Implants 2006; 21: 607-14. 
22. Sykaras N, Lacopino AM, Mrker VA, Triplet RG, woody RD. Implant materials, designs, and surface topographies. Their effect on osseointegration. A literature review. Int J Oral Maxillofacial Implants 2006; 15: 675-90. 
23. Guizzardi S, Galli C, Martini D, Balletti S, Tinti A, Respanti M, et al. Different titanium surface treatment influences human mandibular osteoblast response. J Periodontol 2004; 75: 273-82. 
24.Cochran D. A comparison of endosseous dental implant surfaces. J Periodontol 1999; 70: 1523-39. Quoted from AlSabbagh M. Complications in implant dentistry. Philadelphia: Elsevier, 2015.
25. Kasugai S. Dental implant treatment to osteoporosis patients. Clin Calcium 2006; 16: 348-53. 
26. Shulman LB. Surgical considerations in implant dentistry. J Dent Educ 1988; 52: 712-20. 
27.Chesnut CH, Silverman S, Andriano K, Genant H, Gimona A, Harris S, et al. A randomized trial of nasal spray salmon calcitonin in postmenopausal women with established osteoporosis: the prevent recurrence of osteoporotic fractures study. PROOF Study Group. Am J Med 2000; 109: 267-76. 
28. Pogrel MA, Regezi JA, Harris ST, Goldring SR. Calcitonin treatment for central giant cell granulomas of the mandible: report of two cases. J Oral Maxillofac Surg 1999; 57: 848- 53 
29. Overgaurd K, Hansen MA, Jensen SB, Christensen C. Effect of Salcalcitonin given intranaally on bone mass and fracture rates in established osteoporosis. BMJ 1992; 305: 556-61. 
30. Peichl P, Marteau R, Griesmacher A, Kurapan W, Schedl R, Prosquil E, et al. Salmon calcitonin nasal spray treatment for postmenopausal woman after hip fracture with total hip arthroplasty. J Bone Miner Metab 2005; 23: 243-52. 
31. Greco GD, Las Casas EB, Cornacchia TP, Magalhães CS, Moreira AN. Standard of disocclusion in complete dentures supported by implants without free distal ends: analysis by the finite elements method. J Appl Oral Sci 2012; 20: 64-9. 
32. Fischer JA, Tobler PH, Kaufmann M, Born W, Henke H, Cooper PE, et al. Calcitonin (regional distribution and its binding sites in human brain and pituitary. Proc Natl Acad Sci U S A 1981; 78: 7801-5. 
33. Kaskani E, Lyritis G, Kosmidis C, Galanos A, Andypas G, Chorianopoulos K, et al. Effect of intermittent administration of 200 IU intrasnal salmon calcitonin and low doses of 1 alpha (OH) vitamin D3 on bone mineral density of the lumbar spine and hip region and biochemical bone markers in women with postmenopausal osteoporosis: a pilot study. Clin Rheumatol 2005; 24: 232-8. 
34.Chesnut C, Majumdar S, Newitt D, Shields A, Van Pelt J, Laschansky E, et al. Effects of salmon calcitonin on trabecular microarchitecture as determined by magnetic reasonance imaging: results from the QUEST study. J Bone Miner Res 2005; 20: 1548-61. 
35. Toth E, Csupor E, Meszaros S, Ferencz V, Nemeth L, McCloskey EV, et al. The effect of intranasal salmon calcitonin therapy on bone mineral density in idiophatic male osteoporosis without vertebral fractures-an open label study. Bone 2005; 36: 47-51. 
36. Trovas G, Lyritis G, Galanos A, Raptou P, Constantelou E. A randomized trial of nasal spray salmon calcitonin in men with idiopathic osteoporosis: effects on bone mineral density and bone markers. J Bone Miner Res 2002; 17: 521- 7. 
37.Buclin T, Randin J, Jacquet A, Azria M, Attinge M, Gomez F. The effect of rectal and nasal administration of salmon calcitonin in normal subjects. Calcif Tissue Int 1987; 41: 252-8. 
38. Avioli L. Heterogeneity of osteoporotic syndromes and the response to calcitonin therapy. Calif Tissue Int 1991; 49: 16-9. 

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