EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN ORAL SQUAMOUS CELL CARCINOMA: A CLINICOPATHOLOGICAL STUDY

Document Type : Original Article

Authors

1 BDS 2007, Faculty of Dentistry, Alexandria University

2 - Professor of Oral Pathology, Faculty of Dentistry, Alexandria University

3 Head of oral pathology department, Professor of Oral Pathology, Faculty of Dentistry, Alexandria University

4 Professor of Cranio-Maxillofacial and Plastic Surgery, Faculty of Dentistry, Alexandria University

Abstract

INTRODUCTION: Oral squamous cell carcinoma (OSCC) is one of the most widely occurring cancer worldwide. It represents the tenth most common cancer affecting the world population. Like all other tumors, malignant epithelial cells of the OSCC need adequate blood supply and thus tend to recruit new blood vessels by Angiogenesis (the formation of new vessels by sprouting of the pre-existing endothelium). The major regulators of blood and lymph vessel development are the members of the Vascular Endothelial Growth Factor (VEGF) family. These are multifunctional proteins mainly involved in normal and pathologic angiogenesis. Accordingly, there is an increasing interest in evaluating the diagnostic and prognostic value of VEGF family OBJECTIVES: To evaluate the expression of VEGF-A in OSCC and to correlate it with both histopathological grading and clinical data. MATERIALS AND METHODS: This study includes 20 patients with OSCC. The lesions of concern were clinically examined and biopsied. The tissue biopsies, as well as five negative control specimens, were processed and paraffin sections were prepared. Hematoxylin and eosinstained sections were examined for grading of the carcinoma. The immunohistochemical expression of VEGF was evaluated by the use of Anti-VEGF-A Antibody using the Strept-Avidin-Biotin method on paraffin sections. Immunohistochemical results were evaluated using an image analyzer. Results were recorded and statistically analyzed and correlated with both clinical and histological grading of the tumors. RESULTS: The expression of VEGF was found to be significantly related to the grade of differentiation of the tumor, where the poorer the differentiation, the more the expression the antibody. On the contrary, no significant relation between VEGF expression and clinical data was found. CONCLUSIONS: The expression of VEGF is of a great value as a means of diagnosis concerning histological grading of the tumor, but cannot be used as a sole method for evaluating the case prognosis

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Alexandria Dental Journal
Volume 42, Issue 2
December 2017
Pages 187-192

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