Zohery, A., Nour, Z., Abd El Rehim, S., Mady, M. (2017). HISTOMORPHOMETRIC ANALYSIS OF BONE REGENERATION AFTER USE OF PROPOLIS VERSUS NANOBONE GRAFT MATERIALS FOR THE MANAGEMENT OF CLASS II FURCATION DEFECTS IN DOGS. Alexandria Dental Journal, 42(2), 198-203. doi: 10.21608/adjalexu.2017.57927
Amr A. Zohery; Zubaida M. Nour; Samia S. Abd El Rehim; Marwa I. Mady. "HISTOMORPHOMETRIC ANALYSIS OF BONE REGENERATION AFTER USE OF PROPOLIS VERSUS NANOBONE GRAFT MATERIALS FOR THE MANAGEMENT OF CLASS II FURCATION DEFECTS IN DOGS". Alexandria Dental Journal, 42, 2, 2017, 198-203. doi: 10.21608/adjalexu.2017.57927
Zohery, A., Nour, Z., Abd El Rehim, S., Mady, M. (2017). 'HISTOMORPHOMETRIC ANALYSIS OF BONE REGENERATION AFTER USE OF PROPOLIS VERSUS NANOBONE GRAFT MATERIALS FOR THE MANAGEMENT OF CLASS II FURCATION DEFECTS IN DOGS', Alexandria Dental Journal, 42(2), pp. 198-203. doi: 10.21608/adjalexu.2017.57927
Zohery, A., Nour, Z., Abd El Rehim, S., Mady, M. HISTOMORPHOMETRIC ANALYSIS OF BONE REGENERATION AFTER USE OF PROPOLIS VERSUS NANOBONE GRAFT MATERIALS FOR THE MANAGEMENT OF CLASS II FURCATION DEFECTS IN DOGS. Alexandria Dental Journal, 2017; 42(2): 198-203. doi: 10.21608/adjalexu.2017.57927
HISTOMORPHOMETRIC ANALYSIS OF BONE REGENERATION AFTER USE OF PROPOLIS VERSUS NANOBONE GRAFT MATERIALS FOR THE MANAGEMENT OF CLASS II FURCATION DEFECTS IN DOGS
1Instructor at Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
2Professor of Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
3Professor of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
4Lecturer of Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
Abstract
INTRODUCTION: Nanotechnology has been greatly utilized for bone regeneration strategies. It helps overcome some of the current limitations associated with bone regeneration methods Propolis is a natural substance made by the honeybee, it has effective antioxidant, antimicrobial, and anti-inflammatory effects. Moreover, it has multiple biological effects including improvement of wound healing and bone regeneration. OBJECTIVES: comparison of the effectiveness of NanoBone graft and propolis material on the inter-radicular bone regeneration in Mongrel dogs. MATERIALS AND METHODS: A split mouth design was carried out using the third and fourth mandibular premolars of 3 healthy mongrel dogs. A total of 12 grade II furcation defects were surgically created. The defects in the right side were treated with NanoBone graft and then a collagen membrane was used to cover the defects. In the left side the defects were treated with propolis and then covered with collagen membrane. The dogs were sacrificed after 4 weeks. Segments of the jaw bone containing the defects were then dissected out and prepared for histological evaluation. RESULTS: Both percentage of bone fill and percentage of bone surface area in defects treated with propolis powder were higher than those in NanoBone-filled defects. CONCLUSIONS: Both propolis and NanoBone graft materials have bone regenerative effects with higher ability of propolis to regenerate bone.
1. Papapanou P. Epidemiology of periodontal diseases: an update. Journal of the International Academy of Periodontology. 1999;1:110-6.
2. Petersen PE. The World Oral Health Report 2003: continuous improvement of oral health in the 21st century–the approach of the WHO Global Oral Health Programme. Community Dentistry and oral epidemiology. 2003;31:3-24.
3. Lindhe J, Karring T, Lang NP. Clinical periodontology and implant dentistry: Blackwell Munksgaard Copenhagen; 2003.
4. Kim CS, Choi SH, Cho KS, Chai JK, Wikesjö UM, Kim CK. Periodontal healing in one‐wall intra‐bony defects in dogs following implantation of autogenous bone or a coral‐derived biomaterial. Journal of clinical periodontology. 2005;32:583-9.
5. Ivanovski S, Li H, Daley T, Bartold P. An immunohistochemical study of matrix molecules associated with barrier membrane‐mediated periodontal wound healing. Journal of periodontal research. 2000;35:115-26.
6. Kalra N, Guruprasad C, Naik S, Pradeep A. Stem cells: a novel approach to periodontal regeneration. AOSR. 2011;1:116-21.
7. Al-Sanabani JS, Madfa AA, Al-Sanabani FA. Application of calcium phosphate materials in dentistry. International journal of biomaterials. 2013;2013.
8. Younger EM, Chapman MW. Morbidity at bone graft donor sites. Journal of orthopaedic trauma. 1989;3:192-5.
9. Garrido CA, Lobo SE, Turíbio FM, LeGeros RZ. Biphasic calcium phosphate bioceramics for orthopaedic reconstructions: clinical outcomes. International journal of biomaterials. 2011;2011.
10. Abukawa H, Papadaki M, Abulikemu M, Leaf J, Vacanti JP, Kaban LB, et al. The engineering of craniofacial tissues in the laboratory: a review of biomaterials for scaffolds and implant coatings. Dental Clinics of North America. 2006;50:205-16.
11. Kubinová Š, Syková E. Nanotechnologies in regenerative medicine. Minimally Invasive Therapy & Allied Technologies. 2010;19:144-56.
12. Gerber T, Holzhüter G, Götz W, Bienengräber V, Henkel K-O, Rumpel E. Nanostructuring of biomaterials—a pathway to bone grafting substitute. European Journal of Trauma. 2006;32:132-40.
13. Gerike W, Bienengraber V, Henkel K, Bayerlein T, Proff P, Gedrange T, et al. The manufacture of synthetic nonsintered and degradable bone grafting substitutes. FOLIA MORPHOLOGICA-WARSZAWA-ENGLISH EDITION-. 2006;65:54.
14. Webster TJ, Ergun C, Doremus RH, Siegel RW, Bizios R. Enhanced functions of osteoblasts on nanophase ceramics. Biomaterials. 2000;21:1803-10.
15. GĂśtz W, Lenz S, Reichert C, Henkel K, Pernicka L, Gundlach K, et al. A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig. Folia Histochem Cytobiol. 2011;48:589-98.
16. Toker H, Ozdemir H, Eren K, Ozer H, Sahin G. Nacetylcysteine, a thiol antioxidant, decreases alveolar bone loss in experimental periodontitis in rats. Journal of periodontology. 2009;80:672-8.
17.Bai X-c, Lu D, Bai J, Zheng H, Ke Z-y, Li X-m, et al. Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-κB. Biochemical and biophysical research communications. 2004;314:197-207.
18. Wang X, Stavchansky S, Bowman PD, Kerwin SM. Cytoprotective effect of caffeic acid phenethyl ester (CAPE) and catechol ring-fluorinated CAPE derivatives against menadione-induced oxidative stress in human endothelial cells. Bioorganic & medicinal chemistry. 2006;14:4879-87.
19.Celik S, Gorur S, Aslantas O, Erdogan S, Ocak S, Hakverdi S. Caffeic acid phenethyl ester suppresses oxidative stress in Escherichia coli-induced pyelonephritis in rats. Molecular and cellular biochemistry. 2007;297:131-8.
20. Koru O, Toksoy F, Acikel CH, Tunca YM, Baysallar M, Guclu AU, et al. In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe. 2007;13:140-5.
21.Blonska M, Bronikowska J, Pietsz G, Czuba Z, Scheller S, Krol W. Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A. 1 macrophages. Journal of Ethnopharmacology. 2004;91:25-30.
22. Struillou X, Boutigny H, Badran Z, Fellah BH, Gauthier O, Sourice S, et al. Treatment of periodontal defects in dogs using an injectable composite hydrogel/biphasic calcium phosphate. Journal of Materials Science: Materials in Medicine. 2011;22:1707-17.
24. Ferreira T, Rasband W. ImageJ user guide. IJ1 46r Natl Inst Health, Bethesda. 2012.
25. Ghaitth HM, Lotfy M, Soliman S, Fahmy RA. Quantitative Analysis of Interradicular Bone Regeneration after Use of Modified Perforated Collagen Membrane versus Occlusive Membrane in the Management of Grade II Furcation Defects in Dogs. 2016;5:402-7.
26. Desanctis M, Murphy KG. The role of resective periodontal surgery in the treatment of furcation defects. Periodontology 2000. 2000;22:154-68.
27. Sculean A, Nikolidakis D, Schwarz F. Regeneration of periodontal tissues: combinations of barrier membranes and grafting materials–biological foundation and preclinical evidence: a systematic review. Journal of clinical periodontology. 2008;35:106-16.
28. Shahabuei M, Dabbagh E, Adibrad M, Vaziri S, Eslami B, Afshar SK. The Effect of Platelet-rich Plasma (PRP) and Bio-oss on Bone Regeneration in Furcation Class II Defects: A Histologic and Histomorphometric Study in Dogs. Journal of Dentistry, Shiraz University of Medical Sciences. 2011;12:1-10.
29. Götz W, Gerber T, Michel B, Lossdörfer S, Henkel KO, Heinemann F. Immunohistochemical characterization of nanocrystalline hydroxyapatite silica gel (NanoBone®) osteogenesis: a study on biopsies from human jaws. Clinical oral implants research. 2008;19:1016-26.
30. Ghanaati S, Barbeck M, Willershausen I, Thimm B, Stuebinger S, Korzinskas T, et al. Nanocrystalline hydroxyapatite bone substitute leads to sufficient bone tissue formation already after 3 months: histological and histomorphometrical analysis 3 and 6 months following human sinus cavity augmentation. Clinical implant dentistry and related research. 2013;15:883-92.
31. Ganz C, Xu W, Holzhüter G, Götz W, Vollmar B, Gerber T, editors. Comparison of bone substitutes in a tibia defect model in Wistar-rats. Key Engineering Materials; 2012.
32. Guney A, Karaman I, Oner M, Yerer MB. Effects of propolis on fracture healing: an experimental study. Phytotherapy Research. 2011;25:1648-52.
33. Al-Saeed HF, Mohamed NY. The Possible Therapeutic Effects of Propolis on Osteoporosis in Diabetic Male Rats. Nature and Science 13:136-40.
34. Olczyk P, Wisowski G, Komosinska-Vassev K, Stojko J, Klimek K, Olczyk M, et al. Propolis modifies collagen types I and III accumulation in the matrix of burnt tissue. Evidence-Based Complementary and Alternative Medicine. 2013;2013.
35. Kurek-Górecka A, Rzepecka-Stojko A, Górecki M, Stojko J, Sosada M, Świerczek-Zięba G. Structure and antioxidant activity of polyphenols derived from propolis. Molecules. 2013;19:78-101.