Saleh, M., Darwish, Z., Riad, S., Medra, A. (2015). STUDY OF ODONTOGENIC TUMORS IN THE FACULTY OF DENTISTRY ALEXANDRIA UNIVERSITY AND THE POSSIBLE ROLE OF NESTIN AS A MARKER. Alexandria Dental Journal, 40(2), 180-185. doi: 10.21608/adjalexu.2015.59150
M.M Saleh; Z.E Darwish; S.E Riad; A.M Medra. "STUDY OF ODONTOGENIC TUMORS IN THE FACULTY OF DENTISTRY ALEXANDRIA UNIVERSITY AND THE POSSIBLE ROLE OF NESTIN AS A MARKER". Alexandria Dental Journal, 40, 2, 2015, 180-185. doi: 10.21608/adjalexu.2015.59150
Saleh, M., Darwish, Z., Riad, S., Medra, A. (2015). 'STUDY OF ODONTOGENIC TUMORS IN THE FACULTY OF DENTISTRY ALEXANDRIA UNIVERSITY AND THE POSSIBLE ROLE OF NESTIN AS A MARKER', Alexandria Dental Journal, 40(2), pp. 180-185. doi: 10.21608/adjalexu.2015.59150
Saleh, M., Darwish, Z., Riad, S., Medra, A. STUDY OF ODONTOGENIC TUMORS IN THE FACULTY OF DENTISTRY ALEXANDRIA UNIVERSITY AND THE POSSIBLE ROLE OF NESTIN AS A MARKER. Alexandria Dental Journal, 2015; 40(2): 180-185. doi: 10.21608/adjalexu.2015.59150
STUDY OF ODONTOGENIC TUMORS IN THE FACULTY OF DENTISTRY ALEXANDRIA UNIVERSITY AND THE POSSIBLE ROLE OF NESTIN AS A MARKER
1.Instructor at Oral Pathology Department, Faculty of Dentistry, Alexandria University.
Abstract
originate from epithelium, ectomesenchyme or both. Varying degrees of inductive interaction between these embryonic components of the developing tooth germ exist. Nestin is one of the intermediate filaments constituting the cytoskeleton and is known as a marker of neural stem cells or progenitor cells. Human Nestin expression continues during tooth development and disappears when development is complete. Aim of the work: To study the expression of Nestin in different odontogenic tumors, and to highlight the incidence of odontogenic tumors in the Faculty of dentistry, Alexandria University, Egypt in the past 10 years. Material and Methods: Immunohistochemical analysis was performed on 22 surgical specimens of odontogenic tumors and one control specimen from normal tooth germ.Immunohistochemical staining was performed using a Labeled Strept-Avidin Biotin complex method (LSAB). Results:Almost all the ameloblastomas were negative for Nestin while all cases of odontogenic fibromyxoma , myxoma and odontogenic fibroma showed intense brownish cytoplasmic reaction in both the ectomesenchymal cells and the odontogenic epithelial rests. Ameloblastic fibroma and ameloblastic fibro-odontom cases showed immunopositivity of Nestin in the ameloblast like cells and stellate reticulum like cells as well as the stromal cells. Calcific structures such as dentinoid as well as hamartomatous lesions mainly odontomes were completely negative for Nestin. The results of the statistical analysis revealed higher prevalence of keratocystic odontogenic tumor followed by ameloblastoma. Conclusion:Nestin is expressed in some odontogenic tumors in which the ectomesenchyme of the neural crest origin, constituting a part of their proliferated tissue. In the statistical analysis which was done in this study,there was statistical difference regarding types.
1. Lu Y, Xuan M, Takata T, Wang C, He Z, Zhou Z, et al. Odontogenic tumors. A demographic study of 759 cases in a Chinese population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;86(6):707-14.
2. Mosqueda-Taylor A, Ledesma-Montes C, CaballeroSandoval S, Portilla-Robertson J, Ruiz-Godoy Rivera LM, Meneses-Garcia A. Odontogenic tumors in Mexico: a collaborative retrospective study of 349 cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997;84(6):672-5.
3. El-Gehani R, Orafi M, Elarbi M, Subhashraj K. Benign tumours of orofacial region at Benghazi, Libya: a study of 405 cases. J Craniomaxillofac Surg. 2009;37(7):370-5.
4. Ochsenius G, Ortega A, Godoy L, Penafiel C, Escobar E. Odontogenic tumors in Chile: a study of 362 cases. J Oral Pathol Med. 2002;31(7):415-20.
5. Adebayo ET, Ajike SO, Adekeye EO. A review of 318 odontogenic tumors in Kaduna, Nigeria. J Oral Maxillofac Surg. 2005;63(6):811-9.
6. Lendahl U, Zimmerman LB, McKay RD. CNS stem cells express a new class of intermediate filament protein. Cell. 1990;60(4):585-95.
7. Hendrickson ML, Rao AJ, Demerdash ON, Kalil RE. Expression of nestin by neural cells in the adult rat and human brain. PLoS One. 2011;6(4):e18535.
8. Guerette D, Khan PA, Savard PE, Vincent M. Molecular evolution of type VI intermediate filament proteins. BMC Evol Biol. 2007;7:164.
9. Kachinsky AM, Dominov JA, Miller JB. Myogenesis and the intermediate filament protein, nestin. Dev Biol. 1994;165(1):216-28.
10. Kachinsky AM, Dominov JA, Miller JB. Intermediate filaments in cardiac myogenesis: nestin in the developing mouse heart. J Histochem Cytochem. 1995;43(8):843-7.
11. Frojdman K, Pelliniemi LJ, Lendahl U, Virtanen I, Eriksson JE. The intermediate filament protein nestin occurs transiently in differentiating testis of rat and mouse. Differentiation. 1997;61(4):243-9.
12. Abdel-Rahman A, Rao MS, Shetty AK. Nestin expression in hippocampal astrocytes after injury depends on the age of the hippocampus. Glia. 2004;47(4):299-313.
13. Frisen J, Johansson CB, Torok C, Risling M, Lendahl U. Rapid, widespread, and longlasting induction of nestin contributes to the generation of glial scar tissue after CNS injury. J Cell Biol. 1995;131(2):453-64.
14. Holmin S, Almqvist P, Lendahl U, Mathiesen T. Adult nestin-expressing subependymal cells differentiate to astrocytes in response to brain injury. Eur J Neurosci. 1997;9(1):65-75.
15. Dahlstrand J, Collins VP, Lendahl U. Expression of the class VI intermediate filament nestin in human central nervous system tumors. Cancer Res. 1992;52(19):5334-41.
16. Florenes VA, Holm R, Myklebost O, Lendahl U, Fodstad O. Expression of the neuroectodermal intermediate filament nestin in human melanomas. Cancer Res. 1994;54(2):354-6.
17. Spencer JA, Ferraro F, Roussakis E, Klein A, Wu J, Runnels JM, et al. Direct measurement of local oxygen concentration in the bone marrow of live animals. Nature. 2014;508(7495):269-73.
18. Fujita S, Hideshima K, Ikeda T. Nestin expression in odontoblasts and odontogenic ectomesenchymal tissue of odontogenic tumours. J Clin Pathol. 2006;59(3):240-5.
19. About I, Laurent-Maquin D, Lendahl U, Mitsiadis TA. Nestin expression in embryonic and adult human teeth under normal and pathological conditions. Am J Pathol. 2000;157(1):287-95.
20. About I, Mitsiadis TA. Molecular aspects of tooth pathogenesis and repair: in vivo and in vitro models. Adv Dent Res. 2001;15:59-62.
21. Terling C, Rass A, Mitsiadis TA, Fried K, Lendahl U, Wroblewski J. Expression of the intermediate filament nestin during rodent tooth development. Int J Dev Biol. 1995;39(6):947-56.
22. Wiese C, Rolletschek A, Kania G, Blyszczuk P, Tarasov KV, Tarasova Y, et al. Nestin expression--a property of multi-lineage progenitor cells? Cell Mol Life Sci. 2004;61(19-20):2510-22.
23. Dahlstrand J, Lardelli M, Lendahl U. Nestin mRNA expression correlates with the central nervous system progenitor cell state in many, but not all, regions of developing central nervous system. Brain Res Dev Brain Res. 1995;84(1):109-29.
24. McLachlan JL, Smith AJ, Sloan AJ, Cooper PR. Gene expression analysis in cells of the dentine-pulp complex in healthy and carious teeth. Arch Oral Biol. 2003;48(4):273-83.
25. C. Morsczecka T, W. Gftzb JS, F. Zeilhoferc, U. Ku¨hnb CM, C. Sippela e. Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth. Matrix Biology. 2005;24:155–65.
26. Lobo MVT, Arenas MI, Alonso FJM, Gomez G, Bazan E, Paino e. Nestin, a neuroectodermal stem cell marker molecule, is expressed in Leydig cells of the human testis and in some specific cell types from human testicular tumours. Cell Tissue Res. 2004;316(3):369– 76.
27. Li H, Cherukuri P, Li N, Cowling V, Spinella M, Cole M e. Nestin is expressed in the basal/myoepithelial layer of the mammary gland and is a selective marker of basal epithelial breast tumors. Cancer Res. 2007;67:501–10.
28. Kleeberger W, Bova GS, Nielsen ME, Herawi M, Chuang AY, Epstein JI, et al. Roles for the stem cell associated intermediate filament Nestin in prostate cancer migration and metastasis. Cancer Res. 2007;67(19):9199-206.
29. Ohike N, Sato M, Hisayuki T, Imataka H, Sato S, Wada Y, et al. Immunohistochemical analysis of nestin and ckit and their significance in pancreatic tumors. Pathol Int. 2007;57(9):589-93.
30. Yang X, Wu Q, Yu X, Xu C, Ma B, Zhang X e. Nestin expression in different tumors and its relevance to malignant grade. J Clin Pathol. 2007.volume no.pages ??
31. Philipsen HP, Reichart PA. Classification of odontogenic tumors. A historical review. J Oral Pathology and Medicine. 2006;35:525-9.
32. Takeda Y, T T. Revised histopathological classification of odontogenic tumors by WHO and its allied epithelial cyst. Japanese J of Oral and Maxillofacial Surgery. 2006;52:54-61.
33. Hoffman RM. The potential of nestin-expressing hair follicle stem cells in regenerative medicine. Expert Opin Biol Ther. 2007;7(3):289-91.
34. Teranishi N, Naito Z, Ishiwata T, Tanaka N, Furukawa K, Seya T, et al. Identification of neovasculature using nestin in colorectal cancer. Int J Oncol. 2007;30(3):593- 603.
35. Ebenezer V, Ramalingam B. A cross-sectional survey of prevalence of odontogenic tumours. J Maxillofac Oral Surg. 2010;9(4):369-74.
36. Jing W, Xuan M, Lin Y, Wu L, Liu L, Zheng X, et al. Odontogenic tumours: a retrospective study of 1642 cases in a Chinese population. Int J Oral Maxillofac Surg. 2007;36(1):20-5.
37. Tawfik MA, Zyada MM. Odontogenic tumors in Dakahlia, Egypt: analysis of 82 cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;109(2):e67-73.
38. Osterne RL, Brito RG, Alves AP, Cavalcante RB, Sousa FB. Odontogenic tumors: a 5-year retrospective study in a Brazilian population and analysis of 3406 cases reported in the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;111(4):474-81.
View on SCiNiTO
Top