Asmaa A., H., Gehan M, E., Hanaa M, A., Magda M, S. (2016). THE EFFECT OF LOCAL OXYTOCIN DELIVERY ON HEALING OF BONE DEFECTS IN RABBITS. Alexandria Dental Journal, 41(1), 31-36. doi: 10.21608/adjalexu.2016.59169
Harfoush Asmaa A.; Elba Gehan M; Aly Hanaa M; Saleh Magda M. "THE EFFECT OF LOCAL OXYTOCIN DELIVERY ON HEALING OF BONE DEFECTS IN RABBITS". Alexandria Dental Journal, 41, 1, 2016, 31-36. doi: 10.21608/adjalexu.2016.59169
Asmaa A., H., Gehan M, E., Hanaa M, A., Magda M, S. (2016). 'THE EFFECT OF LOCAL OXYTOCIN DELIVERY ON HEALING OF BONE DEFECTS IN RABBITS', Alexandria Dental Journal, 41(1), pp. 31-36. doi: 10.21608/adjalexu.2016.59169
Asmaa A., H., Gehan M, E., Hanaa M, A., Magda M, S. THE EFFECT OF LOCAL OXYTOCIN DELIVERY ON HEALING OF BONE DEFECTS IN RABBITS. Alexandria Dental Journal, 2016; 41(1): 31-36. doi: 10.21608/adjalexu.2016.59169
THE EFFECT OF LOCAL OXYTOCIN DELIVERY ON HEALING OF BONE DEFECTS IN RABBITS
Demonstrator of Oral Biology - Faculty of Dentistry - Alexandria University
Abstract
INTRODUCTION: Bone defects above critical size do not heal completely by itself and thus represent major clinical challenge to reconstructive surgery. Numerous bone substitutes have already been used to promote bone regeneration. Incorporation of 31biologically active molecules to bone grafts have shown to further improve its bone forming capacity. As a potential candidate for bone regenerating biomolecules which can be used in promoting proliferation and osteogenic differentiation of mesenchymal stem cells, the hypothalamic hormone oxytocin is suggested to be promising. AIM OF THE STUDY: was to evaluate the efficacy of the hypothalamic nonapeptide oxytocin (OT) by direct delivery into local defects using β-tricalcium phosphate (β-TCP) as a carrier. MATERIALS AND METHODS: Twenty one male New Zealand white rabbits weighing 3 Kg (±250 g) were used in this study. Right and left critical size bone defects (CSD) were performed in the edentulous area of rabbit mandibles (diastema). Right side bone defects (Experimental) were filled with OT loaded β-TCP, while the left side bone defects (Control), were filled with saline loaded β-TCP. The effect of OT hormone on bone formation was assessed histologically as well as histomorphometrically after 1, 3 and 6 weeks. RESULTS: Greater amount of new bone formation was noticed in the CSDs filled with the OT loaded groups. The amount of new bone formed was significantly higher during the 3 observational intervals in the OT loaded β-TCP than when compared with the saline loaded β-TCP. CONCLUSION: OT induces new bone formation via an osteoinductive action and can be added to bone grafts following surgical and periodontal surgeries to enhance bone regenerative capacity.
1. Podaropoulos L, Veis AA, Papadimitriou S, Alexandridis C, Kalyvas D. Bone regeneration using btricalcium phosphate in a calcium sulfate matrix. Journal of Oral Implantology. 2009;35(1):28-36.
2. Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000;21(24):2529-43.
3. Jang ES, Park JW, Kweon H, Lee KG, Kang SW, Baek D-H, et al. Restoration of peri-implant defects in immediate implant installations by Choukroun plateletrich fibrin and silk fibroin powder combination graft. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2010;109(6):831-6.
4. Giannoudis PV, Dinopoulos H, Tsiridis E. Bone substitutes: an update. Injury. 2005;36(3):S20-S7.
5. Varghese S, Phadke A. Synthetic bone grafts. Google Patents; 2014.
6. Clarke S, Brooks R, Lee P, Rushton N. The effect of osteogenic growth factors on bone growth into a ceramic filled defect around an implant. Journal of Orthopaedic Research. 2004;22(5):1016-24.
7. Clarke S, Hoskins N, Jordan G, Henderson S, Marsh D. In vitro testing of Advanced JAX™ Bone Void Filler System: species differences in the response of bone marrow stromal cells to β tri-calcium phosphate and carboxymethylcellulose gel. Journal of Materials Science: Materials in Medicine. 2007;18(12):2283-90.
8. Rodriguez R, Kondo H, Nyan M, Hao J, Miyahara T, Ohya K, et al. Implantation of green tea catechin α‐ tricalcium phosphate combination enhances bone repair in rat skull defects. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2011;98(2):263- 71.
9. Xu L, Lv K, Zhang W, Zhang X, Jiang X, Zhang F. The healing of critical-size calvarial bone defects in rat with rhPDGF-BB, BMSCs, and β-TCP scaffolds. Journal of Materials Science: Materials in Medicine. 2012;23(4):1073-84.
10. Elabd C, Basillais A, Beaupied H, Breuil V, Wagner N, Scheideler M, et al. Oxytocin controls differentiation of human mesenchymal stem cells and reverses osteoporosis. Stem Cells. 2008;26(9):2399-407.
11. Colli VC, Okamoto R, Spritzer PM, Dornelles RCM. Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats. Archives of Oral Biology. 2012;57(9):1290-7.
12. Colaianni G, Tamma R, Di Benedetto A, Yuen T, Sun L, Zaidi M, et al. The Oxytocin–Bone Axis. Journal of Neuroendocrinology. 2014;26(2):53-7.
13. Colaianni G, Sun L, Di Benedetto A, Tamma R, Zhu LL, Cao J, et al. Bone marrow oxytocin mediates the anabolic action of estrogen on the skeleton. Journal of Biological Chemistry. 2012;287(34):29159-67.
14. Elabd S, Sabry I, Hassan W, Nour H, Zaky K. Possible neuroendocrine role for oxytocin in bone remodeling. Endocrine Regulations. 2007;41(4):131-41
15. Tamma R, Colaianni G, Zhu Ll, DiBenedetto A, Greco G, Montemurro G, et al. Oxytocin is an anabolic bone hormone. Proceedings of the National Academy of Sciences. 2009;106(17):7149-54.
16. Park JW, Kim JM, Lee HJ, Jeong SH, Suh JY, Hanawa T. Bone healing with oxytocin‐loaded microporous β‐ TCP bone substitute in ectopic bone formation model and critical‐sized osseous defect of rat. Journal of Clinical Periodontology. 2014;41(2):181-90.
17. Eleftheriadis E, Leventis MD, Tosios KI, Faratzis G, Titsinidis S, Eleftheriadi I, et al. Osteogenic activity of. BETA.-tricalcium phosphate in a hydroxyl sulphate matrix and demineralized bone matrix: a histological study in rabbit mandible. Journal of Oral Science. 2010;52(3):377-84.
18. Yugoshi LI, Sala MA, Brentegani LG, Carvalho TLL. Histometric study of socket healing after tooth extraction in rats treated with diclofenac. Brazilian Dental Journal. 2002;13(2):92-6.
19. Habibovic P, Yuan H, van der Valk CM, Meijer G, van Blitterswijk CA, de Groot K. 3D microenvironment as essential element for osteoinduction by biomaterials. Biomaterials. 2005;26(17):3565-75.
20. Breuil V, Panaia-Ferrari P, Fontas E, Roux C, Kolta S, Eastell R, et al. Oxytocin, a new determinant of bone mineral density in post-menopausal women: analysis of the OPUS cohort. The Journal of Clinical Endocrinology & Metabolism. 2014;99(4):E634-E41.
21. MacIntyre I, Zaidi M, Alam A, Datta HK, Moonga BS, Lidbury PS, et al. Osteoclastic inhibition: an action of nitric oxide not mediated by cyclic GMP. Proceedings of the National Academy of Sciences. 1991;88(7):2936- 40.
22. Cattaneo M, Chini B, Vicentini L. Oxytocin stimulates migration and invasion in human endothelial cells. British Journal of Pharmacology. 2008;153(4):728-36.
View on SCiNiTO
Top